School of Health and Life Sciences, Post-Graduate Program in Dentistry, Pontifical Catholic University of Rio Grande do Sul, PUCRS, Avenida Ipiranga, 6681, Partenon, Porto Alegre, RS, 90619-900, Brazil.
School of Medicine, Post-Graduate Program in Medicine and Health Sciences, Pontifical Catholic University of Rio Grande do Sul, PUCRS, Porto Alegre, RS, Brazil.
Oral Maxillofac Surg. 2024 Mar;28(1):355-361. doi: 10.1007/s10006-023-01151-9. Epub 2023 Apr 5.
After extraction, dental alveolus filling aims to reduce bone loss and maintain the alveolus volume during patient rehabilitation. Boric acid (BA) is a boron-derived compound with osteogenic properties and an interesting candidate for alveoli filling. This study aims to investigate the osteogenic capacity of the local application of BA in dental socket preservation.
Thirty-two male Wistar rats were submitted to upper right incisor extraction and randomly divided into four groups (n = 8): control group (no intervention), BA (8 mg/kg) socket filling, bone graft (Cerabone®, Botiss, Germany), and BA + bone graft socket filling. Animals were euthanized 28 days after dental extraction. MicroCT and histological analysis were performed to evaluate the newly formed bone on the dental alveolus.
MicroCT analysis demonstrated that bone volume fraction (BV/TV), bone surface (BS), bone surface/bone volume ratio (BS/BV), bone surface density (BS/TV), trabecular thickness (Tb.Th), total bone porosity (Po-tot), and total volume of pore space (Po.V(tot)) from BA and BA + bone graft rats were significantly different from the control group. Histological evaluation displayed a delayed bone repair in BA rats, with the presence of connective tissue and inflammatory infiltrate. However, the BA + bone graft group demonstrated histological aspects like the bone graft animals, with less organized osteoblasts, suggesting inferior bone repair.
Osteogenic capacity did not depend on the BA local application after 28 days of dental extraction. The presence of inflammation in the BA group can represent toxicity induced by the substance dosage used.
拔牙后，牙槽骨填充的目的是减少骨丢失并在患者康复期间维持牙槽骨体积。硼酸（BA）是一种具有成骨特性的硼衍生化合物，是牙槽骨填充的一个有趣候选物。本研究旨在探讨局部应用 BA 对牙槽窝保存的成骨能力。
32 只雄性 Wistar 大鼠进行右上切牙拔除，并随机分为四组（n = 8）：对照组（无干预）、BA（8mg/kg）牙槽窝填充、骨移植（德国 Botiss 的 Cerabone®）和 BA + 骨移植牙槽窝填充。拔牙后 28 天处死动物。进行 microCT 和组织学分析以评估新形成的牙槽骨。
microCT 分析表明，BA 和 BA + 骨移植大鼠的骨体积分数（BV/TV）、骨表面（BS）、骨表面/骨体积比（BS/BV）、骨表面密度（BS/TV）、骨小梁厚度（Tb.Th）、总骨孔隙率（Po-tot）和总孔空间体积（Po.V(tot)）与对照组有显著差异。组织学评价显示 BA 组的骨修复延迟，存在结缔组织和炎症浸润。然而，BA + 骨移植组表现出类似于骨移植动物的组织学特征，骨细胞排列较差，提示骨修复较差。
在拔牙后 28 天，局部应用 BA 对成骨能力没有影响。BA 组的炎症存在可能代表所用物质剂量引起的毒性。
