Kang Hyuckjoon, Cabrera Janel R, Zee Barry M, Kang Heather A, Jobe Jenny Marie, Hegarty Maeve B, Barry Aurelie E, Glotov Alexander, Schwartz Yuri B, Kuroda Mitzi I
Division of Genetics, Brigham and Women's Hospital, Boston, MA 02115, USA.
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
Sci Adv. 2022 Sep 9;8(36):eadd0103. doi: 10.1126/sciadv.add0103. Epub 2022 Sep 7.
Polycomb group (PcG) mutants were first identified in on the basis of their failure to maintain proper gene repression during development. The proteins encoded by the corresponding fly genes mainly assemble into one of two discrete Polycomb repressive complexes: PRC1 or PRC2. However, biochemical analyses in mammals have revealed alternative forms of PRC2 and multiple distinct types of noncanonical or variant PRC1. Through a series of proteomic analyses, we identify analogous PRC2 and variant PRC1 complexes in , as well as a broader repertoire of interactions implicated in early development. Our data provide strong support for the ancient diversity of PcG complexes and a framework for future analysis in a longstanding and versatile genetic system.
多梳蛋白家族（PcG）突变体最早于[具体时间]被鉴定出来，其依据是它们在发育过程中无法维持适当的基因抑制。相应果蝇基因编码的蛋白质主要组装成两种离散的多梳抑制复合物之一：PRC1或PRC2。然而，哺乳动物中的生化分析揭示了PRC2的替代形式以及多种不同类型的非经典或变体PRC1。通过一系列蛋白质组学分析，我们在[具体物种]中鉴定出类似的PRC2和变体PRC1复合物，以及与早期发育相关的更广泛的相互作用。我们的数据为PcG复合物的古老多样性提供了有力支持，并为在一个长期且通用的遗传系统中进行未来分析提供了框架。
