Henderson J T, Nieman R A, Bieber A L
Biochim Biophys Acta. 1987 Aug 5;914(2):152-61. doi: 10.1016/0167-4838(87)90058-6.
The 400 MHz 1H-NMR spectrum of myotoxin a from the venom of Crotalus viridis viridis is described. The identification of spin systems in the aromatic region corresponding to the six aromatic residues of myotoxin a was completed using both one- and two-dimensional NMR spectroscopy and the pH dependence of chemical shifts. Assignments of these spin systems to specific residues was possible for the singly occurring amino acids Tyr-1 and Phe-12. Resonances from Tyr-1, His-5 and His-10 were shifted significantly from their random coil values in a pH-dependent manner. These shift perturbations were deemed evidence of a helical arrangement of the amino terminal region which placed these residues in close proximity to each other.
描述了来自绿曼巴蛇毒液的肌毒素a的400 MHz 1H-NMR光谱。利用一维和二维NMR光谱以及化学位移的pH依赖性，完成了在芳香区对应于肌毒素a六个芳香族残基的自旋系统的鉴定。对于单个出现的氨基酸Tyr-1和Phe-12，可以将这些自旋系统指定给特定残基。Tyr-1、His-5和His-10的共振在pH依赖性方式下从其无规卷曲值有显著位移。这些位移扰动被认为是氨基末端区域呈螺旋排列的证据，该排列使这些残基彼此靠近。
