College of Traditional Chinese Medicine, Jinan University, Guangzhou, China.
Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.
Phytother Res. 2023 Jan;37(1):151-162. doi: 10.1002/ptr.7603. Epub 2022 Sep 7.
This study explored the therapeutic effect of α-asarone on chronic sciatica. Thirty-two Sprague-Dawley (SD) rats were divided into four groups: the sham group, chronic constriction injury (CCI) group, pregabalin group, and α-asarone group. Hot hyperalgesia was induced after the CCI operation, and α-asarone was found to relieve chronic neuralgia. Furthermore, α-asarone reduced IL1β, IL6, TNF-α, CRP, and LPS levels and increased IL10 levels in serum. α-Asarone decreased the protein levels of TRPA1, TRPM8, and TRPV1-4 and the mRNA levels of TRPA1, TRPM8, TRPV1-4, IL1β, and TNF-α in dorsal root ganglion neurons. In the sciatic nerve, α-asarone treatment reduced the number of inflammatory cells and promoted the proliferation of Schwann cells, favouring recovery of the nerve structure. In cellular experiments, LPS induced Schwann cell apoptosis via TLR4/p38MAPK signalling; α-asarone attenuated LPS-induced Schwann cell apoptosis by decreasing TLR4, p-p38MAPK, cleaved-caspase3, and cleaved-caspase7 levels and increasing Bcl-2 and Bcl-xl expression. Overall, these findings suggest that α-asarone relieves chronic sciatica by decreasing the levels of inflammatory factors, inhibiting peripheral sensitization, and favouring the repair of damaged nerves.
本研究探讨了α-细辛脑对慢性坐骨神经痛的治疗作用。32 只 SD 大鼠分为四组：假手术组、慢性压迫损伤（CCI）组、普瑞巴林组和α-细辛脑组。CCI 手术后诱导热痛觉过敏，发现α-细辛脑缓解慢性神经痛。此外，α-细辛脑降低了血清中 IL1β、IL6、TNF-α、CRP 和 LPS 水平，增加了 IL10 水平。α-细辛脑降低了背根神经节神经元中 TRPA1、TRPM8 和 TRPV1-4 的蛋白水平和 TRPA1、TRPM8、TRPV1-4、IL1β 和 TNF-α的 mRNA 水平。在坐骨神经中，α-细辛脑治疗减少了炎症细胞的数量，并促进施万细胞的增殖，有利于神经结构的恢复。在细胞实验中，LPS 通过 TLR4/p38MAPK 信号诱导施万细胞凋亡；α-细辛脑通过降低 TLR4、p-p38MAPK、cleaved-caspase3 和 cleaved-caspase7 水平，增加 Bcl-2 和 Bcl-xl 表达，减轻 LPS 诱导的施万细胞凋亡。总的来说，这些发现表明，α-细辛脑通过降低炎症因子水平、抑制外周敏化和促进受损神经修复来缓解慢性坐骨神经痛。
