Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Aichi 444-8787, Japan.
J Reprod Dev. 2022 Oct 6;68(5):340-344. doi: 10.1262/jrd.2022-003. Epub 2022 Sep 7.
The present study established techniques to induce pseudopregnancy, in vitro oocyte cultures from pronuclear to 2- to 4-cell stages, and embryo transfer in musk shrews, a reflex ovulator. Offspring were subsequently obtained by transferring in vivo-developed or in vitro-cultured embryos. Female musk shrews received human chronic gonadotropin (hCG), with or without mating stimuli, from vasectomized males to produce pseudopregnant recipients. Embryos at the 2- to 4-cell stage were collected 44-48 h after mating. Another set of embryos was collected 26-27 h after mating and then cultured for 20 h from the pronuclear to 2- to 4-cell stages. Subsequently, embryos were transferred into the oviducts of pseudopregnant recipients 24 or 48 h after the induction of pseudopregnancy. Offsprings were successfully obtained from recipients that received hCG 24 h before embryo transfer, regardless of mating stimuli. These techniques may be valuable for producing transgenic musk shrews.
本研究建立了诱导假孕、体外原核期至 2-4 细胞期卵母细胞培养以及胚胎移植的技术，从而在反射排卵的麝鼠中获得后代。通过移植体内发育或体外培养的胚胎获得后代。雌性麝鼠接受来自输精管切除术雄性的人绒毛膜促性腺激素（hCG），有或没有交配刺激，以产生假孕受体。在交配后 44-48 小时收集 2-4 细胞期的胚胎。另一组胚胎在交配后 26-27 小时收集，然后从原核期培养至 2-4 细胞期 20 小时。随后，在诱导假孕后 24 或 48 小时将胚胎移植到假孕受体的输卵管中。无论是否有交配刺激，接受 hCG 24 小时前胚胎移植的受体都成功获得了后代。这些技术对于生产转基因麝鼠可能具有重要价值。
