球状蛋白质的热变性。酰胺III光谱区域的傅里叶变换红外研究。
Thermal denaturation of globular proteins. Fourier transform-infrared studies of the amide III spectral region.
作者信息
Anderle G, Mendelsohn R
出版信息
Biophys J. 1987 Jul;52(1):69-74. doi: 10.1016/S0006-3495(87)83189-2.
Abstract
Fourier transform-infrared (FT-IR) spectra are reported for the amide III spectral region of the native and thermally denatured forms of chymotrypsinogen, ribonuclease, bovine serum albumin, and lysozyme. Chymotrypsinogen denatures into structures containing substantial contributions from beta-sheets, while lysozyme and bovine serum albumin show increased amounts of random-coil forms. The changes observed for ribonuclease are quite small. Bovine serum albumin shows at least six bands in the 1,260-1,320 cm-1 region which undergo large intensity changes upon thermal denaturation, and hence are assignable to alpha-helical amide III modes. The large number of observed bands suggests that slight variations in helical geometry, symmetry, or interactions result in changed amide III frequencies, so that simple correlations between narrow frequency ranges and secondary structures may not be applicable for this mode. A widened frequency range is suggested as diagnostic for helical structures.
摘要
报道了胰凝乳蛋白酶原、核糖核酸酶、牛血清白蛋白和溶菌酶天然形式和热变性形式的酰胺III光谱区域的傅里叶变换红外（FT-IR）光谱。胰凝乳蛋白酶原变性为含有大量β-折叠贡献的结构，而溶菌酶和牛血清白蛋白显示出增加的无规卷曲形式的量。核糖核酸酶观察到的变化非常小。牛血清白蛋白在1260 - 1320 cm-1区域至少有六个条带，这些条带在热变性时经历大的强度变化，因此可归因于α-螺旋酰胺III模式。观察到的大量条带表明，螺旋几何形状、对称性或相互作用的轻微变化会导致酰胺III频率改变，因此窄频率范围与二级结构之间的简单相关性可能不适用于此模式。建议拓宽频率范围作为螺旋结构的诊断依据。
相似文献
1
Thermal denaturation of globular proteins. Fourier transform-infrared studies of the amide III spectral region.球状蛋白质的热变性。酰胺III光谱区域的傅里叶变换红外研究。
Biophys J. 1987 Jul;52(1):69-74. doi: 10.1016/S0006-3495(87)83189-2.
2
Identification of beta-turn and random coil amide III infrared bands for secondary structure estimation of proteins.用于蛋白质二级结构估计的β-转角和无规卷曲酰胺III红外波段的识别。
Biophys Chem. 1999 Jul 19;80(1):7-20. doi: 10.1016/s0301-4622(99)00060-5.
3
Fourier transform infrared spectrometric analysis of protein conformation: effect of sampling method and stress factors.蛋白质构象的傅里叶变换红外光谱分析：采样方法和应激因素的影响
Anal Biochem. 2001 Oct 15;297(2):160-9. doi: 10.1006/abio.2001.5337.
4
Estimation of beta-structure content of proteins by means of deconvolved FTIR spectra.通过去卷积傅里叶变换红外光谱法估算蛋白质的β-结构含量
J Biochem Biophys Methods. 1985 Oct;11(4-5):235-40. doi: 10.1016/0165-022x(85)90005-3.
5
Solvent denaturation of proteins as observed by resolution-enhanced Fourier transform infrared spectroscopy.
J Biochem Biophys Methods. 1984 Jul;9(3):193-9. doi: 10.1016/0165-022x(84)90024-1.
6
Secondary structure and temperature-induced unfolding and refolding of ribonuclease T1 in aqueous solution. A Fourier transform infrared spectroscopic study.核糖核酸酶T1在水溶液中的二级结构及温度诱导的去折叠和重折叠：傅里叶变换红外光谱研究
J Mol Biol. 1993 Aug 5;232(3):967-81. doi: 10.1006/jmbi.1993.1442.
7
Pressure effects on protein secondary structure and hydrogen deuterium exchange in chymotrypsinogen: a Fourier transform infrared spectroscopic study.压力对胰凝乳蛋白酶原二级结构及氢氘交换的影响：傅里叶变换红外光谱研究
Biochim Biophys Acta. 1988 Aug 31;956(1):1-9. doi: 10.1016/0167-4838(88)90291-9.
8
Protein secondary structures in water from second-derivative amide I infrared spectra.基于二阶导数酰胺I红外光谱的水中蛋白质二级结构
Biochemistry. 1990 Apr 3;29(13):3303-8. doi: 10.1021/bi00465a022.
9
Two-dimensional mid-IR and near-IR correlation spectra of ribonuclease A: using overtones and combination modes to monitor changes in secondary structure.核糖核酸酶A的二维中红外和近红外相关光谱：利用泛音和组合模式监测二级结构变化
Biospectroscopy. 1998;4(5 Suppl):S19-29. doi: 10.1002/(SICI)1520-6343(1998)4:5+<S19::AID-BSPY3>3.0.CO;2-N.
10
Secondary structure of 11 S globulin in aqueous solution investigated by FT-IR derivative spectroscopy.
Biochim Biophys Acta. 1988 Nov 23;957(2):272-80. doi: 10.1016/0167-4838(88)90283-x.
引用本文的文献
1
Interaction of the lysozyme with anticoagulant drug warfarin: Spectroscopic and computational analyses.溶菌酶与抗凝血药物华法林的相互作用：光谱学和计算分析。
Heliyon. 2024 May 7;10(10):e30818. doi: 10.1016/j.heliyon.2024.e30818. eCollection 2024 May 30.
2
Rapid and Label-Free Histopathology of Oral Lesions Using Deep Learning Applied to Optical and Infrared Spectroscopic Imaging Data.利用深度学习对光学和红外光谱成像数据进行口腔病变的快速无标记组织病理学分析
J Pers Med. 2024 Mar 13;14(3):304. doi: 10.3390/jpm14030304.
3
Tailoring the Techno-Functional Properties of Fava Bean Protein Isolates: A Comparative Evaluation of Ultrasonication and Pulsed Electric Field Treatments.定制蚕豆分离蛋白的技术功能特性：超声处理和脉冲电场处理的比较评估
Foods. 2024 Jan 24;13(3):376. doi: 10.3390/foods13030376.
4
Probing the interaction of lysozyme with cardiac glycoside digitoxin: experimental and analyses.探究溶菌酶与强心苷地高辛的相互作用：实验与分析
Front Mol Biosci. 2023 Dec 22;10:1327740. doi: 10.3389/fmolb.2023.1327740. eCollection 2023.
5
Insight into Organization of Gliadin and Glutenin Extracted from Gluten Modified by Phenolic Acids.从酚酸改性的面筋中提取的麦醇溶蛋白和麦谷蛋白的结构解析。
Molecules. 2023 Nov 27;28(23):7790. doi: 10.3390/molecules28237790.
6
Light-Induced Conformational Alterations in Heliorhodopsin Triggered by the Retinal Excited State.视紫红质中由视网膜激发态引发的光诱导构象变化。
J Phys Chem B. 2021 Aug 12;125(31):8797-8804. doi: 10.1021/acs.jpcb.1c04551. Epub 2021 Aug 3.
7
Effects of Physical and Chemical Factors on the Structure of Gluten, Gliadins and Glutenins as Studied with Spectroscopic Methods.物理和化学因素对谷蛋白、麦醇溶蛋白和麦谷蛋白结构的影响的光谱研究。
Molecules. 2021 Jan 19;26(2):508. doi: 10.3390/molecules26020508.
8
Spectroscopic and Molecular Docking Investigation on the Noncovalent Interaction of Lysozyme with Saffron Constituent "Safranal".溶菌酶与藏红花成分“藏红花醛”非共价相互作用的光谱和分子对接研究
ACS Omega. 2020 Apr 16;5(16):9131-9141. doi: 10.1021/acsomega.9b04291. eCollection 2020 Apr 28.
9
Raman spectroscopy for the diagnosis of unlabeled and unstained histopathological tissue specimens.用于诊断未标记和未染色组织病理学标本的拉曼光谱技术。
World J Gastrointest Oncol. 2018 Nov 15;10(11):439-448. doi: 10.4251/wjgo.v10.i11.439.
10
Influence of luminescent graphene quantum dots on trypsin activity.荧光石墨烯量子点对胰蛋白酶活性的影响。
Int J Nanomedicine. 2018 Mar 15;13:1525-1538. doi: 10.2147/IJN.S155021. eCollection 2018.
本文引用的文献
1
AN ANALYSIS OF THE OPTICAL ROTATORY DISPERSION OF POLYPEPTIDES AND PROTEINS.多肽与蛋白质的旋光色散分析
Proc Natl Acad Sci U S A. 1964 Apr;51(4):695-702. doi: 10.1073/pnas.51.4.695.
2
Infrared membrane spectroscopy.红外膜光谱法。
Mol Biol Biochem Biophys. 1981;31:270-332. doi: 10.1007/978-3-642-81537-9_6.
3
Vibrational analysis of peptides, polypeptides, and proteins. XVIII. Conformational sensitivity of the alpha-helix spectrum: alpha I- and alpha II-poly(L-alanine).肽、多肽和蛋白质的振动分析。十八。α-螺旋光谱的构象敏感性：αI-和αII-聚（L-丙氨酸）。
Biopolymers. 1984 May;23(5):923-43. doi: 10.1002/bip.360230509.
4
Laser-excited Raman spectroscopy of biomolecules. I. Native lysozyme and its constituent amino acids.生物分子的激光激发拉曼光谱。I. 天然溶菌酶及其组成氨基酸。
J Mol Biol. 1970 Jun 14;50(2):509-24. doi: 10.1016/0022-2836(70)90208-1.
5
A new basis for interpreting the circular dichroic spectra of proteins.一种解释蛋白质圆二色光谱的新依据。
Proc Natl Acad Sci U S A. 1971 May;68(5):969-72. doi: 10.1073/pnas.68.5.969.
6
Raman studies of the helix-to-coil transition in poly-L-glutamic acid and poly-L-ornithine.
Biopolymers. 1972;11(9):1871-92. doi: 10.1002/bip.1972.360110908.
7
Laser-excited Raman spectroscopy of biomolecules. 3. Native bovine serum albumin and beta-lactoglobulin.
Biochim Biophys Acta. 1972 Feb 29;257(2):280-7. doi: 10.1016/0005-2795(72)90280-2.
8
Raman scattering of native and thermally denatured lysozyme.
Biochim Biophys Acta. 1972 Jun 22;271(1):16-22. doi: 10.1016/0005-2795(72)90128-6.
9
Laser-excited Raman spectroscopy of biomolecules. V. Conformational changes associated with the chemical denaturation of lysozyme.生物分子的激光激发拉曼光谱。V. 与溶菌酶化学变性相关的构象变化
J Am Chem Soc. 1974 May 15;96(10):3038-42. doi: 10.1021/ja00817a003.
10
Intensities and other spectral parameters of infrared amide bands of polypeptides in the beta- and random forms.β折叠和无规形式多肽的红外酰胺带的强度及其他光谱参数。
Biopolymers. 1973 Jun;12(6):1337-51. doi: 10.1002/bip.1973.360120610.
Zotero 插件