Wang Baoquan, Yin Changjun, Yang Xu, Shi Huibo, Zhang Zheng, Zhou Jun, Zhang Peitong
Department of Oncology, China Aacdemy of Chinese Medical Sciences Guang'anmen Hospital, Beijing 100053, China.
Graduate School, Beijing University of Chinese Medicine, Beijing 100029, China.
Evid Based Complement Alternat Med. 2022 Aug 29;2022:4304361. doi: 10.1155/2022/4304361. eCollection 2022.
The aim of the study is to explore the relationship between lymphatic metastasis genes, prognosis, and immune cell infiltration in patients with colon cancer.
Based on the Cancer Genome Atlas Program (TCGA) database, differentially expressed genes and prognostic genes related to colon adenocarcinoma (COAD) lymphatic metastasis were screened and intersected. We used lasso and univariate Cox regression analysis to screen core genes and establish a preliminary prediction model. GO and KEGG enrichment analysis was used for lymphatic metastasis-related genes, and single GSEA was used for the final screening results. Finally, we evaluated the relationship between identified genes and immune cell infiltration.
A total of 1727 genes were differentially expressed between COAD patients with TNM stages of N0 and N1. After further screening, six core genes (RNU4-2, ZNF556, RNVU1-15, NSA2P6, RN7SL767P, and RN7SL473P) were obtained, and a preliminary prediction model was established, in which ZNF556 was a risk factor, and the rest were protective factors. Single GSEA showed that pathways such as systemic lupus erythematosus might play an important role in the initial lymphatic metastasis of COAD. GO and KEGG enrichment analysis of 1727 genes supported this result. Immune infiltration analysis showed that six genes were significantly correlated with T cell and NK cell families.
Six core genes may affect COAD initial lymphatic metastasis through the systemic lupus erythematosus pathway and immune cell infiltration.
本研究旨在探讨结肠癌患者淋巴转移基因、预后与免疫细胞浸润之间的关系。
基于癌症基因组图谱计划（TCGA）数据库，筛选并交叉分析与结肠腺癌（COAD）淋巴转移相关的差异表达基因和预后基因。我们使用套索回归和单变量Cox回归分析来筛选核心基因并建立初步预测模型。对淋巴转移相关基因进行GO和KEGG富集分析，对最终筛选结果进行单样本基因集富集分析（GSEA）。最后，我们评估了所鉴定基因与免疫细胞浸润之间的关系。
在TNM分期为N0和N1的COAD患者之间，共有1727个基因存在差异表达。进一步筛选后，获得了六个核心基因（RNU4-2、ZNF556、RNVU1-15、NSA2P6、RN7SL767P和RN7SL473P），并建立了初步预测模型，其中ZNF556是危险因素，其余为保护因素。单样本GSEA显示，系统性红斑狼疮等通路可能在COAD的初始淋巴转移中起重要作用。对1727个基因的GO和KEGG富集分析支持了这一结果。免疫浸润分析表明，六个基因与T细胞和NK细胞家族显著相关。
六个核心基因可能通过系统性红斑狼疮通路和免疫细胞浸润影响COAD的初始淋巴转移。
