Department of Life Sciences, National Chung Hsing University, Taichung City, Taiwan.
Department of Life Sciences, National Chung Hsing University, Taichung City; Translational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, Taichung City, Taiwan.
Chin J Physiol. 2022 Jul-Aug;65(4):209-214. doi: 10.4103/cjp.cjp_78_21.
Antrodia salmonea (AS) is a fungus, which belongs to a fungal family of Taiwanofungus salmoneus with the features of anti-oxidant, anti-inflammatory, and anticancer. Recent studies have shown that AS has anti-cancer functions in ovarian and breast cancer. However, the effects of AS on prostate cancer (PCa) proliferation remain unknown. Therefore, we investigated the role of AS in PCa proliferation through apoptosis, and cell cycle regulation in PCa cell lines. Our results showed that Antrodia salmonea extract (ASE) inhibited PCa cells growth with a dose-dependent manner. In addition, ASE decreased the anchorage-independent growth formation ability in PC3 cells. Moreover, ASE-induced cell growth inhibition in PCa cells (DU145, PC3) was correlated to decreased cell cycle-related proteins such as cyclin A/B and cyclin-dependent kinase CDK1/2/4, and increased cell cycle inhibitor proteins p21. Besides, ASE decreased the total protein level of epidermal growth factor receptor and its downstream signaling pathways Akt and Erk in both PCa cells. We found that apoptotic markers such as cleaved-PARP protein levels increased significantly in DU145 cells indicating ASE might induce apoptosis. In conclusion, our results suggest that ASE may have the ability to induce PCa cell death through regulating cell cycle arrest and apoptosis pathways.
樟芝（AS）是一种真菌，属于台湾拟青霉属的真菌家族，具有抗氧化、抗炎和抗癌作用。最近的研究表明，AS 对卵巢癌和乳腺癌具有抗癌作用。然而，AS 对前列腺癌（PCa）增殖的影响尚不清楚。因此，我们通过细胞凋亡和细胞周期调控研究了 AS 在 PCa 细胞系中的增殖作用。我们的结果表明，樟芝提取物（ASE）以剂量依赖性方式抑制 PCa 细胞生长。此外，ASE 降低了 PC3 细胞中的锚定非依赖性生长形成能力。此外，ASE 诱导的 PCa 细胞（DU145、PC3）生长抑制与细胞周期相关蛋白（如细胞周期蛋白 A/B 和细胞周期蛋白依赖性激酶 CDK1/2/4）减少和细胞周期抑制剂蛋白 p21 增加有关。此外，ASE 降低了两种 PCa 细胞中表皮生长因子受体及其下游信号通路 Akt 和 Erk 的总蛋白水平。我们发现凋亡标志物如裂解 PARP 蛋白水平在 DU145 细胞中显著增加，表明 ASE 可能诱导细胞凋亡。总之，我们的结果表明，ASE 可能通过调节细胞周期阻滞和细胞凋亡途径诱导 PCa 细胞死亡。
