Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, College of Food Science and Engineering, Nanjing University of Finance and Economics, Wenyuan Road 3, Nanjing, Jiangsu, 210023, China.
Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, College of Food Science and Engineering, Nanjing University of Finance and Economics,Wenyuan Road 3,Nanjing, Jiangsu, 210023, China.
FEMS Microbiol Lett. 2022 Oct 14;369(1). doi: 10.1093/femsle/fnac090.
Fungi often experience oxidative stress in response to the environment during growth. In this study, Aspergillus niger HY2, whose presence easily results in paddy mildew, was used to investigate the effects of different carbon sources on morphological development, reactive oxygen species (ROS) metabolites, and antioxidant enzymes activities. Morphological development mainly includes the changes in conidial production and colony diameter. ROS metabolites production include the content of hydrogen peroxide (H2O2), superoxide anion (O2·-), and malondialdehyde (MDA). The results indicated that A. niger HY2 adapted to H2O2 exposure by decreasing growth and increasing the activities of some antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). Different carbon sources also affected the expression of the developmental-specific gene flbA and the oxidative stress tolerance-related gene cat. When incubated with glucose, sucrose, and xylose as carbon sources, A. niger exhibited stronger oxidative stress tolerance, but when incubated with maltose as a carbon source, A. niger exhibited relatively poor oxidative stress tolerance. Our results can provide a theoretical basis for further understanding mechanisms of metabolic adaptation and developing targeted strategies to control the spoilage caused by A. niger.
真菌在生长过程中经常会因环境而经历氧化应激。在这项研究中，使用容易导致水稻赤霉病的黑曲霉 HY2 来研究不同碳源对形态发育、活性氧（ROS）代谢物和抗氧化酶活性的影响。形态发育主要包括产孢量和菌落直径的变化。ROS 代谢物的产生包括过氧化氢（H2O2）、超氧阴离子（O2·-）和丙二醛（MDA）的含量。结果表明，黑曲霉 HY2 通过降低生长速度和增加一些抗氧化酶（包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx））的活性来适应 H2O2 暴露。不同的碳源也会影响发育特异性基因 flbA 和氧化应激耐受相关基因 cat 的表达。当以葡萄糖、蔗糖和木糖作为碳源时，黑曲霉表现出更强的氧化应激耐受能力，但当以麦芽糖作为碳源时，黑曲霉表现出相对较差的氧化应激耐受能力。我们的研究结果可以为进一步理解代谢适应的机制以及开发针对黑曲霉引起的腐败的控制策略提供理论基础。
