Midbrain reticular stimulation produces patterns of metabolic activation and suppression in the cerebellum and vestibular nuclei: a 2-deoxyglucose study.

Autoradiographic 2-deoxyglucose (2-DG) procedures were used to map the activity in the cerebellum and vestibular nuclei during electrical stimulation of the midbrain reticular formation (MRF) in unrestrained rats. The major finding was a large increase in 2-DG uptake observed in the flocculus of MRF-stimulated rats. The peak of labeling in the flocculus was greater than any other peak of labeling measured in the cerebellum of MRF-stimulated or control rats. Structures showing significant decreases in 2-DG uptake included the 3 deep cerebellar nuclei and the 3 vestibular nuclei. The most pronounced suppressive effects of MRF stimulation were on the medial and lateral vestibular nuclei. The changes in metabolic activity revealed by 2-DG provide a first anatomical demonstration of: the activating effects of MRF stimulation on the flocculus; and the suppressive effects of MRF stimulation on deep cerebellar and vestibular nuclei. The observed patterns of metabolic activation and suppression were correlated with the known electrophysiological properties of the structures affected by MRF stimulation. The findings are consistent with specific effects of MRF stimulation on floccular-vestibular-visual interactions that may be disruptive to learning functions such as adaptability of the vestibulo-ocular reflex. The effects of MRF stimulation on the deep cerebellar nuclei are also consistent with a potential disruption of somatomotor learning-related activities in these nuclei. The results support the existence of MRF mechanisms for the modulation of integrative sensory-motor functions in the cerebellum.