Brillard J P, Galut O, Nys Y
Br Poult Sci. 1987 Jun;28(2):307-18. doi: 10.1080/00071668708416963.
Spermatozoa incubated in uterine fluid collected 7 or 18 h after ovulation showed no significant differences either in motility or in fecundity, despite wide variations of composition of the uterine fluid itself. The absence of uterine fluid in the oviduct 1 h before oviposition may be partially responsible for spermatozoa being unable to migrate easily to the storage sites after insemination of this time. Females inseminated intravaginally at the presumed time of oviposition showed consistently low fertility, irrespective of whether an egg was present in the uterus or not. Normal fertility rates could be achieved with inseminations intravaginally at or near the time of oviposition if the uterine contractions associated with oviposition were inhibited by treatment with indomethacin. Hens inseminated intravaginally 1 h after oviposition retained lower proportions (0.4 to 0.7%) of the initial dose of spermatozoa (measured 2 h after insemination) in their oviduct that hens inseminated 5 to 6 h after oviposition (4.5 to 23.3%).
在排卵后7小时或18小时收集的子宫液中孵育的精子，无论子宫液本身的成分差异有多大，其活力和受精能力均无显著差异。产卵前1小时输卵管中没有子宫液，这可能是导致在此期间输精后精子无法轻松迁移到储存部位的部分原因。在假定的产卵时间经阴道输精的母鸡，无论子宫内是否有蛋，其生育力始终较低。如果用吲哚美辛治疗抑制与产卵相关的子宫收缩，在产卵时或接近产卵时经阴道输精可实现正常的生育率。与在产卵后5至6小时输精的母鸡（4.5%至23.3%）相比，在产卵后1小时经阴道输精的母鸡在输卵管中保留的初始精子剂量比例较低（0.4%至0.7%，输精后2小时测量）。
