Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
School of Pharmacy, Chengdu University, Chengdu, 610106, Sichuan, China.
Sci Data. 2022 Sep 8;9(1):552. doi: 10.1038/s41597-022-01668-w.
The chicken provides large amounts of protein for the human diet and is also used as a model organism for biomedical research. Increasing meat production is an important goal in the poultry industry and skeletal muscles have highly diverse origins, shapes, metabolic features, and physical functions. Previous gene expression atlases have largely ignored the differences among diverse types of skeletal muscles; therefore, comprehensive transcriptional maps of all skeletal muscles are needed to improve meat production traits. In this study, we sequenced 58 samples from 10 different skeletal muscles of 42-day-old White Plymouth Rock chickens. We also measured myofiber diameter and generated myofiber-type datasets of these 10 tissues. We generated 418.4 Gb high-quality bulk RNA-Seq data from four or six biological replicates of each skeletal muscle (four replicates from extraocular samples) (approximately 7.4 Gb per sample). This dataset provides valuable information for understanding the muscle fiber characteristics of White Plymouth Rock chickens. Furthermore, our data can be used as a model for heterogeneity analysis between tissues with similar properties.
鸡肉为人类饮食提供了大量的蛋白质,并且也被用作生物医学研究的模式生物。增加肉类产量是家禽业的一个重要目标,而骨骼肌具有高度多样化的起源、形状、代谢特征和物理功能。以前的基因表达图谱在很大程度上忽略了不同类型骨骼肌之间的差异;因此,需要对所有骨骼肌进行全面的转录图谱绘制,以改善肉质生产性状。在这项研究中,我们对 42 日龄白洛克鸡的 10 种不同骨骼肌中的 58 个样本进行了测序。我们还测量了肌纤维直径,并生成了这 10 种组织的肌纤维型数据集。我们从每个骨骼肌的四个或六个生物学重复中(来自眼外样本的四个重复)生成了 418.4GB 高质量的 bulk RNA-Seq 数据(每个样本约 7.4GB)。该数据集为了解白洛克鸡的肌肉纤维特征提供了有价值的信息。此外,我们的数据可以用作具有相似特性的组织之间异质性分析的模型。