无标记定量蛋白质组学揭示了微波诱导苦荞萌发和黄酮类化合物富集的机制。
Label-free quantitative proteomics reveals the mechanism of microwave-induced Tartary buckwheat germination and flavonoids enrichment.
机构信息
College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, Anhui, China.
College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, Anhui, China.
出版信息
Food Res Int. 2022 Oct;160:111758. doi: 10.1016/j.foodres.2022.111758. Epub 2022 Jul 30.
The objective of this study was to reveal the mechanism underlying the effects of microwave treatment on Tartary buckwheat germination and flavonoids enrichment. Label-free quantitative proteomic analysis showed that Tartary buckwheat germinated at 3, 5, and 7 days after 300 W/50 s microwave treatment had 7, 5, and 5 differentially expressed proteins (DEPs) compared to those of control. These DEPs are mainly related to energy production and conversion, gene expression, and flavonoids metabolism. Based on KEGG analysis, the DEPs were mainly enriched in photosynthesis, RNA polymerase, flavonoid biosynthesis, phenylalanine metabolism, and phenylpropanoid biosynthesis metabolic pathways. Further, the upregulation of phenylalanine ammonia-lyase and flavonol synthase protein enzymes promoted germination and flavonoids accumulation in Tartary buckwheat. These findings reveal the mechanism of Tartary buckwheat germination and the enrichment of flavonoids induced by microwaves and provide a scientific basis for the development of functional foods for Tartary buckwheat.
本研究旨在揭示微波处理对苦荞发芽和黄酮类化合物富集的作用机制。非标记定量蛋白质组学分析表明，与对照组相比，经 300 W/50 s 微波处理后在第 3、5 和 7 天发芽的苦荞分别有 7、5 和 5 个差异表达蛋白（DEPs）。这些 DEPs 主要与能量产生和转化、基因表达以及黄酮类化合物代谢有关。基于 KEGG 分析，DEPs 主要富集在光合作用、RNA 聚合酶、黄酮类生物合成、苯丙氨酸代谢和苯丙烷类生物合成代谢途径中。此外，苯丙氨酸解氨酶和类黄酮合酶蛋白酶的上调促进了苦荞的发芽和黄酮类化合物的积累。这些发现揭示了微波诱导苦荞发芽和黄酮类化合物富集的机制，为苦荞功能性食品的开发提供了科学依据。
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