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比较分析苦荞种子在三个发育阶段的蛋白质组学。

Comparative proteomic analyses of Tartary buckwheat (Fagopyrum tataricum) seeds at three stages of development.

机构信息

Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang, 550001, China.

College of Life Science, Sichuan Agricultural University, Ya'an, 625014, China.

出版信息

Funct Integr Genomics. 2022 Dec;22(6):1449-1458. doi: 10.1007/s10142-022-00912-1. Epub 2022 Nov 11.

DOI:10.1007/s10142-022-00912-1
PMID:36369301
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9701650/
Abstract

Tartary buckwheat is among the valuable crops, utilized as both food and Chinese herbal medicine. To uncover the accumulation dynamics of the main nutrients and their regulatory mechanism of Tartary buckwheat seeds, microscopic observations and nutrient analysis were conducted which suggested that starch, proteins as well as flavonoid gradually accumulated among seed development. Comparative proteomic analysis of rice Tartary buckwheat at three different developmental stages was performed. A total of 78 protein spots showed differential expression with 74 of them being successfully identified by MALDI-TOF/TOF MS. Among them, granule bound starch synthase (GBSS1) might be the critical enzyme that determines starch biosynthesis, while 11 S seed storage protein and vicilin seemed to be the main globulin and affect seed storage protein accumulation in Tartary buckwheat seeds. Two enzymes, flavanone 3-hydroxylase (F3H) and anthocyanidin reductase (ANR), involved in the flavonoid biosynthesis pathway were identified. Further analysis on the expression profiles of flavonoid biosynthetic genes revealed that F3H might be the key enzyme that promote flavonoid accumulation. This study provides insights into the mechanism of nutrition accumulation at the protein level in Tartary buckwheat seeds and may facilitate in the breeding and enhancement of Tartary buckwheat germplasm.

摘要

苦荞麦是一种珍贵的作物，既可以作为食物，也可以作为中草药。为了揭示苦荞麦种子主要营养物质的积累动态及其调控机制，进行了微观观察和营养分析，结果表明淀粉、蛋白质和类黄酮在种子发育过程中逐渐积累。对三个不同发育阶段的水稻苦荞麦进行了比较蛋白质组学分析。共鉴定出 78 个差异表达蛋白点，其中 74 个蛋白点通过 MALDI-TOF/TOF MS 成功鉴定。其中，颗粒结合淀粉合成酶（GBSS1）可能是决定淀粉生物合成的关键酶，而 11S 种子贮藏蛋白和伴大豆球蛋白似乎是主要球蛋白，并影响苦荞麦种子中贮藏蛋白的积累。鉴定出两种参与类黄酮生物合成途径的酶，即黄烷酮 3-羟化酶（F3H）和花青素还原酶（ANR）。对类黄酮生物合成基因表达谱的进一步分析表明，F3H 可能是促进类黄酮积累的关键酶。本研究为苦荞麦种子在蛋白质水平上的营养积累机制提供了新的见解，可能有助于苦荞麦种质的培育和改良。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a2f/9701650/9238aa4868e2/10142_2022_912_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a2f/9701650/f02d9d11addb/10142_2022_912_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a2f/9701650/d9b2bd97098d/10142_2022_912_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a2f/9701650/287f1d54d3af/10142_2022_912_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a2f/9701650/58835dace8e2/10142_2022_912_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a2f/9701650/8589a74cf8aa/10142_2022_912_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a2f/9701650/9238aa4868e2/10142_2022_912_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a2f/9701650/f02d9d11addb/10142_2022_912_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a2f/9701650/d9b2bd97098d/10142_2022_912_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a2f/9701650/287f1d54d3af/10142_2022_912_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a2f/9701650/58835dace8e2/10142_2022_912_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a2f/9701650/8589a74cf8aa/10142_2022_912_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a2f/9701650/9238aa4868e2/10142_2022_912_Fig6_HTML.jpg

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2
Comparative metabolomics study of Tartary (Fagopyrum tataricum (L.) Gaertn) and common (Fagopyrum esculentum Moench) buckwheat seeds.鞑靼荞麦和普通荞麦种子的比较代谢组学研究。
Food Chem. 2022 Mar 1;371:131125. doi: 10.1016/j.foodchem.2021.131125. Epub 2021 Sep 15.
3
Milling fractions composition of common (Fagopyrum esculentum Moench) and Tartary (Fagopyrum tataricum (L.) Gaertn.) buckwheat.
多组学鉴定参与苦荞芦丁水解的关键糖苷水解酶基因 FtGH1。
Plant Biotechnol J. 2024 May;22(5):1206-1223. doi: 10.1111/pbi.14259. Epub 2023 Dec 8.
4
Exploring and targeting potential druggable antimicrobial resistance targets ArgS, SecY, and MurA in Staphylococcus sciuri with TCM inhibitors through a subtractive genomics strategy.运用消减基因组学策略，从中药抑制剂中探寻并针对金黄色酿脓葡萄球菌 ArgS、SecY 和 MurA 等潜在可成药的抗微生物耐药性靶标。
Funct Integr Genomics. 2023 Jul 26;23(3):254. doi: 10.1007/s10142-023-01179-w.
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Food Chem. 2021 Dec 15;365:130459. doi: 10.1016/j.foodchem.2021.130459. Epub 2021 Jun 25.
4
Genome-wide identification of bZIP transcription factor genes related to starch synthesis in barley ( L.).大麦（L.）中与淀粉合成相关的bZIP转录因子基因的全基因组鉴定。
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The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality.胚乳特异性转录因子 TaNAC019 调控谷蛋白和淀粉积累，其优良等位基因可提高小麦籽粒品质。
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