Faculty of Electrical Engineering, University of Ljubljana, 1000 Ljubljana, Slovenia.
Faculty of Electronics, Vilnius Gediminas Technical University, 03227 Vilnius, Lithuania.
Int J Mol Sci. 2022 Aug 23;23(17):9543. doi: 10.3390/ijms23179543.
A high-intensity pulsed electromagnetic field (HI-PEMF) is a non-invasive and non-contact delivery method and may, as such, have an advantage over gene electrotransfer mediated by conventional electroporation using contact electrodes. Due to the limited number of in vitro studies in the field of gene electrotransfection by HI-PEMF, we designed experiments to investigate and demonstrate the feasibility of such a technique for the non-viral delivery of genetic material into cells in vitro. We first showed that HI-PEMF causes DNA adsorption to the membrane, a generally accepted prerequisite step for successful gene electrotransfection. We also showed that HI-PEMF can induce gene electrotransfection as the application of HI-PEMF increased the percentage of GFP-positive cells for two different combinations of pDNA size and concentration. Furthermore, by measuring the uptake of larger molecules, i.e., fluorescently labelled dextrans of three different sizes, we showed endocytosis to be a possible mechanism for introducing large molecules into cells by HI-PEMF.
高强度脉冲电磁场（HI-PEMF）是一种非侵入性和非接触式的传递方法，因此可能优于使用接触电极的传统电穿孔介导的基因电转移。由于 HI-PEMF 介导的基因电转染的体外研究数量有限，我们设计了实验来研究和证明这种技术将遗传物质非病毒递送到体外细胞的可行性。我们首先表明，HI-PEMF 导致 DNA 吸附到细胞膜上，这是基因电转染成功的公认前提步骤。我们还表明，HI-PEMF 可以诱导基因电转染，因为随着 HI-PEMF 的应用增加，两种不同大小和浓度的 pDNA 的 GFP 阳性细胞的百分比增加。此外，通过测量较大分子（即三种不同大小的荧光标记葡聚糖）的摄取，我们表明内吞作用可能是 HI-PEMF 将大分子引入细胞的一种机制。
