The Innate Immune Response Group, IdiPAZ, La Paz University Hospital, 28046 Madrid, Spain.
Tumour Immunology Lab, IdiPAZ, La Paz University Hospital, 28046 Madrid, Spain.
Int J Mol Sci. 2022 Aug 26;23(17):9672. doi: 10.3390/ijms23179672.
Human-adipose-derived mesenchymal stem cells (hADMSCs) are multipotent stem cells which have become of great interest in stem-cell therapy due to their less invasive isolation. However, they have limited migration and short lifespans. Therefore, understanding the mechanisms by which these cells could migrate is of critical importance for regenerative medicine. Looking for novel alternatives, herein, hADMSCs were isolated from adipose tissue and co-cultured with human monocytes ex vivo. A new fused hybrid entity, a foam hybrid cell (FHC), which was CD90CD14, resulted from this co-culture and was observed to have enhanced motility, proliferation, immunomodulation properties, and maintained stemness features. Our study demonstrates the generation of a new hybrid cellular population that could provide migration advantages to MSCs, while at the same time maintaining stemness properties.
人脂肪间充质干细胞（hADMSCs）是多能干细胞，由于其非侵入性的分离方法，在干细胞治疗中受到了极大的关注。然而，它们的迁移能力有限，寿命也较短。因此，了解这些细胞迁移的机制对于再生医学至关重要。为了寻找新的替代方法，本研究从脂肪组织中分离 hADMSCs 并进行体外共培养与人类单核细胞。这种共培养产生了一种新的融合杂交实体，即泡沫杂交细胞（FHC），它表达 CD90CD14，并观察到具有增强的迁移、增殖、免疫调节特性，并且保持了干细胞特征。我们的研究证明了一种新的杂交细胞群体的产生，它可以为间充质干细胞提供迁移优势，同时保持干细胞特性。
