Lin Nan-Chin, Shih Yin-Hwa, Chiu Kuo-Chou, Li Po-Jung, Yang Hui-Wu, Lan Wan-Chen, Hsia Shih-Min, Wang Tong-Hong, Shieh Tzong-Ming
Department of Oral and Maxillofacial Surgery, Show Chwan Memorial Hospital, Changhua 500009, Taiwan.
Department of Oral and Maxillofacial Surgery, Changhua Christian Hospital, Changhua 500, Taiwan.
Cancers (Basel). 2022 Aug 30;14(17):4217. doi: 10.3390/cancers14174217.
The polypeptide rs9679162 and mRNA expression were associated with treatment outcome in various cancers. However, the relation of and head and neck cancer were nuclear. A total of 199 patients with head and neck squamous cell carcinoma (HNSCC) were collected in this study, including oral SCC (OSCC), oropharyngeal SCC (OPSCC), laryngeal SCC (LSCC), and others. The DNA and RNA of cancer tissues were extracted using the TRI Reagent method. The rs9679162 was analyzed using polymerase chain reaction (PCR) and sequencing methods in 199 DNA specimens, and the mRNA expression was analyzed using quantitative reverse transcription PCR (RT-qPCR) methods in 68 paired RNA specimens of non-cancerous matched tissues (NCMT) and tumor tissues. The results showed that the genotype of TT, TG, and GG appeared at 30%, 44%, and 26%, respectively. Non-TT genotype or G alleotype were associated with alcohol, betel nut, and cigarette using among patients with OSCC, and it also affected the treatment and survival of patients with OSCC and LSCC. High mRNA expression levels increased lymphatic metastasis of patients with HNSCC, and treatment and survival in patients with OPSCC. Overall, the -rs9679162 genotype and mRNA expression level can be used as indicators of HNSCC treatment prognosis.
多肽rs9679162和mRNA表达与多种癌症的治疗结果相关。然而，其与头颈癌的关系是核心的。本研究共收集了199例头颈部鳞状细胞癌（HNSCC）患者，包括口腔鳞状细胞癌（OSCC）、口咽鳞状细胞癌（OPSCC）、喉鳞状细胞癌（LSCC）等。采用TRI试剂法提取癌组织的DNA和RNA。对199份DNA标本采用聚合酶链反应（PCR）和测序方法分析rs9679162，对68对非癌匹配组织（NCMT）和肿瘤组织的RNA标本采用定量逆转录PCR（RT-qPCR）方法分析mRNA表达。结果显示，TT、TG和GG基因型分别占30%、44%和26%。非TT基因型或G等位基因型与OSCC患者饮酒、嚼槟榔和吸烟有关，也影响OSCC和LSCC患者的治疗和生存。高mRNA表达水平增加了HNSCC患者的淋巴转移，以及OPSCC患者的治疗和生存。总体而言，-rs9679162基因型和mRNA表达水平可作为HNSCC治疗预后的指标。
