牛脑钙结合蛋白的鉴定
Identification of bovine brain calcium binding proteins.
作者信息
Tokuda M, Khanna N C, Waisman D M
出版信息
Cell Calcium. 1987 Jun;8(3):229-39. doi: 10.1016/0143-4160(87)90021-2.
Three peaks of calcium binding activity have been identified by the Chelex-100 calcium binding assay of the fractions from DEAE cellulose chromatography of 100,000 X g supernatant of bovine brain. These calcium binding activity peaks have been subjected to extensive purification and three novel calcium binding proteins (Mr 27,000, Mr 48,000 and Mr 63,000) and two previously characterized proteins (calcineurin and calmodulin) have been identified as components of calcium binding activity peaks. Analysis of the calcium binding properties of the novel proteins by equilibrium dialysis suggests these proteins may be intracellular calcium receptors.
通过对牛脑100,000×g上清液进行DEAE纤维素层析得到的各组分进行Chelex - 100钙结合测定，已鉴定出三个钙结合活性峰。这些钙结合活性峰已进行了广泛的纯化，并且已鉴定出三种新型钙结合蛋白（分子量分别为27,000、48,000和63,000）以及两种先前已表征的蛋白（钙调神经磷酸酶和钙调蛋白）作为钙结合活性峰的组成成分。通过平衡透析对这些新型蛋白的钙结合特性进行分析表明，这些蛋白可能是细胞内钙受体。
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