Department of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, 20133 Milano, Italy.
Department of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, 26900 Lodi, Italy.
Nutrients. 2022 Sep 5;14(17):3666. doi: 10.3390/nu14173666.
γ-Conglutin (γ-C) is the glycoprotein from the edible seed , studied for long time for its postprandial glycaemic regulating action. It still lacks clear information on what could happen at the meeting point between the protein and the organism: the intestinal barrier. We compared an in vitro system involving Caco-2 and IPEC-J2 cells with an ex vivo system using pig ileum and jejunum segments to study γ-C transport from the apical to the basolateral compartment, and its effects on the D-glucose uptake and glucose transporters protein expression. Finally, we studied its potential in modulating glucose metabolism by assessing the possible inhibition of α-amylase and α-glucosidase. RP-HPLC analyses showed that γ-C may be transported to the basolateral side in the in vitro system but not in the pig intestines. γ-C was also able to promote a decrease in glucose uptake in both cells and jejunum independently from the expression of the SGLT1 and GLUT2 transporters.
γ-伴大豆球蛋白（γ-C）是可食用种子中的糖蛋白，其对餐后血糖的调节作用已被长期研究。但对于该蛋白在与机体的交汇点（肠道屏障）处可能会发生什么，目前仍缺乏明确的信息。我们比较了体外 Caco-2 和 IPEC-J2 细胞系统与使用猪回肠和空肠段的离体系统，以研究 γ-C 从顶端到基底外侧腔的转运及其对 D-葡萄糖摄取和葡萄糖转运蛋白表达的影响。最后，我们通过评估对α-淀粉酶和α-葡萄糖苷酶的可能抑制作用，研究了其对葡萄糖代谢的潜在调节作用。反相高效液相色谱分析表明，γ-C 可能在体外系统中被转运到底侧，但在猪肠中不能。γ-C 还能够独立于 SGLT1 和 GLUT2 转运体的表达，促进细胞和空肠中葡萄糖摄取的减少。
