Baruwa Abayomi Omokeji, Mazzitelli Claudia, Maravic Tatjana, Martins Jorge N R, Mazzoni Annalisa, Ginjeira António
Department of Endodontics, Faculdade de Medicina Dentária, Universidade de Lisboa, Rua Professora Teresa Ambrósio, 1600-277 Lisboa, Portugal.
Department of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna, Via S. Vitale 59, 40125 Bologna, Italy.
Polymers (Basel). 2022 Aug 30;14(17):3567. doi: 10.3390/polym14173567.
The objective was to evaluate the effect of different root canal irrigating solutions on the activity of matrix metalloproteinases (MMPs), and correlation to the push-out bond strength (PBS) and nanoleakage expression (NL) in the root dentin. Seventy-two single-rooted teeth were treated endodontically and distributed into four groups (n = 6 for in-situ zymography, n = 10 for PBS, and n = 2 for NL per group) according to the irrigating solutions used: (I) saline (S); (II) 5.25% sodium hypochlorite (SH); (III) 5.25% SH + 10% citric acid (CA); and (IV) 5.25% SH + 10% CA + 0.2% chlorhexidine (CHX). After root canal obturation, post space was prepared to receive the glass fiber post. Dual-cure resin was used for luting and light polymerization was performed. The root/fiber post assemblies were sectioned and subjected to in situ zymography, and PBS and NL expression analysis tests. The enzymatic activity was quantified and expressed as a percentage of the green fluorescence, while fractographic evaluation was performed after PBS with a stereomicroscope, and data were statistically analyzed at p < 0.05. The zymography analysis shows high expression of MMPs in the middle third of the root in all groups, while the most abundant activity of MMPs following the irrigating solutions is observed in groups I and III, where saline and citric acid are used, respectively. Inversely, group IV, where chlorhexidine is the final rinse, records the lowest MMP activity with the highest PBS, and the statistical analysis of the groups are ranked as: IV > II > III > I (p < 0.05). The combination of SH, CA, and CHX results in lower expression of MMPs and higher push-out bond strength of fiber posts to root dentin, with no difference seen in the nanoleakage expression (p > 0.05); hence, this irrigation regime with chlorhexidine as a final rinse is more favorable than other combinations in ensuring optimal adhesion to root dentine.
本研究旨在评估不同根管冲洗液对基质金属蛋白酶（MMPs）活性的影响，以及其与根管牙本质中推出粘结强度（PBS）和纳米渗漏表达（NL）的相关性。选取72颗单根管牙进行根管治疗，并根据所使用的冲洗液分为四组（原位酶谱每组n = 6，PBS每组n = 10，NL每组n = 2）：（I）生理盐水（S）；（II）5.25%次氯酸钠（SH）；（III）5.25% SH + 10%柠檬酸（CA）；（IV）5.25% SH + 10% CA + 0.2%氯己定（CHX）。根管充填后，制备桩道以容纳玻璃纤维桩。采用双固化树脂粘结并进行光固化。将牙根/纤维桩组件切片，进行原位酶谱分析以及PBS和NL表达分析测试。酶活性通过绿色荧光百分比进行定量，PBS测试后使用体视显微镜进行断口分析评估，数据进行统计学分析，p < 0.05。酶谱分析显示所有组牙根中三分之一处MMPs表达较高，而分别使用生理盐水和柠檬酸的I组和III组在冲洗液处理后MMPs活性最为丰富。相反，以氯己定作为最终冲洗液的IV组记录到最低的MMP活性和最高的PBS，各组的统计学分析排名为：IV > II > III > I（p < 0.05）。SH、CA和CHX联合使用可降低MMPs表达，并提高纤维桩与根管牙本质的推出粘结强度，纳米渗漏表达无差异（p > 0.05）；因此，以氯己定作为最终冲洗液的这种冲洗方案在确保与根管牙本质的最佳粘结方面比其他组合更有利。
