使用液相色谱-串联质谱法优化CYP抑制试验。
Optimization of the CYP inhibition assay using LC-MS/MS.
作者信息
Abduraman Muhammad Asyraf, Mustafa Nor Hidayah, Yaacob Nik Soriani, Amanah Azimah, Tan Mei Lan
机构信息
School of Pharmaceutical Sciences, Universiti Sains Malaysia, Pulau Pinang 11800, Malaysia.
Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia.
出版信息
MethodsX. 2022 Aug 23;9:101827. doi: 10.1016/j.mex.2022.101827. eCollection 2022.
The data presented in this article are related to the research article entitled "Cytochrome P450 inhibition activities of non-standardized botanical products" [1], in which the possible CYP inhibitory properties of botanical products were investigated. This article describes the optimization and bioanalytical method validation of the CYP (Cytochrome P450 inhibition assay) inhibition assays, namely, phenacetin O-deethylase assay, testosterone 6β-hydroxylase assay, felodipine dehydrogenase assay and midazolam 1'-hydroxylase assay using LC-MS/MS.
本文所呈现的数据与题为《非标准化植物产品的细胞色素P450抑制活性》的研究论文[1]相关，该论文对植物产品可能的CYP抑制特性进行了研究。本文描述了使用LC-MS/MS对CYP（细胞色素P450抑制试验）抑制试验（即非那西丁O-脱乙基酶试验、睾酮6β-羟化酶试验、非洛地平脱氢酶试验和咪达唑仑1'-羟化酶试验）的优化及生物分析方法验证。
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