Sakuma R, Nishina T, Kitamura M
Clin Chem. 1987 Aug;33(8):1427-30.
We evaluated six deproteinizing methods for determination of uric acid in serum by "high-performance" liquid chromatography with ultraviolet detection: those involving zinc hydroxide, sodium tungstate, trichloroacetic acid, perchloric acid, acetonitrile, and centrifugal ultrafiltration (with Amicon MPS-1 devices). We used a Toyosoda ODS-120A reversed-phase column. The mobile phase was sodium phosphate buffer (40 mmol/L, pH 2.2) containing 20 mL of methanol per liter. Absorbance of the eluate was monitored at 284 nm. The precipitation method with perchloric acid gave high recoveries of uric acid and good precision, and results agreed with those by the uricase-catalase method of Kageyama (Clin Chim Acta 1971;31:421-6).
我们评估了六种用于通过带紫外检测的“高效”液相色谱法测定血清中尿酸的脱蛋白方法：这些方法涉及氢氧化锌、钨酸钠、三氯乙酸、高氯酸、乙腈和离心超滤（使用密理博MPS-1装置）。我们使用了东洋曹达ODS-120A反相柱。流动相为每升含20 mL甲醇的磷酸钠缓冲液（40 mmol/L，pH 2.2）。在284 nm处监测洗脱液的吸光度。高氯酸沉淀法得到了较高的尿酸回收率和良好的精密度，结果与影山的尿酸酶-过氧化氢酶法（《临床化学学报》1971年；31：421 - 426）一致。
