USDA ARS Sugarcane Research Unit, 5883 Usda Rd., Houma, LA, USA70360-5578.
USDA ARS Honey Bee Breeding, Genetics and Physiology Laboratory, 1157 Ben Hur Rd., Baton Rouge, LA, USA70820-5502.
Biol Open. 2022 Oct 15;11(10). doi: 10.1242/bio.059039. Epub 2022 Sep 29.
Food quantity and macronutrients contribute to honey bee health and colony survival by mediating immune responses. We determined if this held true for bees injected with chronic bee paralysis virus (CBPV) and deformed wing virus (DWV), two common honey bee ssRNA viruses. Pollen-substitute diet and syrup consumption rates and macronutrient preferences of two Varroa-resistant stocks (Pol-Line and Russian bees) were compared to Varroa-susceptible Italian bees. Bee stocks varied in consumption, where Italian bees consumed more than Pol-Line and Russian bees. However, the protein: lipid (P:L) ratios of diet consumed by the Italian and Russian bees was greater than that of the Pol-Line bees. Treatment had different effects on consumption based on the virus injected. CBPV was positively correlated with syrup consumption, while DWV was not correlated with consumption. P:L ratios of consumed diet were significantly impacted by the interaction of bee stock and treatment, with the trends differing between CBPV and DWV. Variation in macronutrient preferences based on viral species may indicate differences in energetic costs associated with immune responses to infections impacting different systems. Further, virus species interacted with bee genotype, indicating different mechanisms of viral resistance or tolerance among honey bee genotypes.
食物数量和宏量营养素通过调节免疫反应来促进蜜蜂健康和蜂群生存。我们确定这是否适用于注射慢性麻痹病毒（CBPV）和变形翅膀病毒（DWV）的蜜蜂，这两种病毒是常见的蜜蜂 ssRNA 病毒。我们比较了两种抗瓦螨的蜂种（波兰线蜂和俄罗斯蜂）与易感瓦螨的意大利蜂的花粉替代饲料和糖浆消耗率以及宏量营养素偏好。蜂群的消耗情况有所不同，其中意大利蜂的消耗多于波兰线蜂和俄罗斯蜂。然而，意大利蜂和俄罗斯蜂消耗的饲料中蛋白质：脂肪（P:L）比例大于波兰线蜂。根据注射的病毒，处理对消耗有不同的影响。CBPV 与糖浆消耗呈正相关，而 DWV 与消耗无关。消耗的饲料的 P:L 比例受到蜂种和处理的相互作用的显著影响，CBPV 和 DWV 之间的趋势不同。基于病毒种类的宏量营养素偏好的变化可能表明与感染免疫反应相关的能量成本存在差异，从而影响不同的系统。此外，病毒种类与蜜蜂基因型相互作用，表明不同的蜜蜂基因型对病毒具有不同的抗性或耐受性机制。
