Collaborative Innovation Center for Child Nutrition and Health Development, Chongqing Engineering Research Center of Functional Food, Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing, 400067, China.
Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital & Chongqing Cancer Institute & Chongqing Cancer Hospital, Chongqing, 400030, China.
Probiotics Antimicrob Proteins. 2023 Oct;15(5):1371-1381. doi: 10.1007/s12602-022-09991-9. Epub 2022 Sep 9.
Functional constipation is one of the most common gastrointestinal disorders. Oxidative stress can aggravate organ dysfunction. Enteric neurotransmitters have significant effects on the regulation of the enteric nervous system and intestinal muscle contraction. Oxidative stress and reduced gastrointestinal motility are considered to be one of the main causes of constipation. This study aimed to investigate whether LimosiLactobacillus pentosus CQZC02 alleviated loperamide hydrochloride (Lop)-induced constipation in mice under high-fat diet (HFD) conditions and to elucidate the underlying mechanism, focusing on enteric neurotransmitters. Four-week-old female BALB/c mice were randomly divided into five groups: normal group (Nor), constipation model group (H-Lop), L. pentosus CQZC02 low-dose group (H-Lop + ZC02L), L. pentosus CQZC02 high-dose group (H-Lop + ZC02H), and LimosiLactobacillus bulgaricus control group (H-Lop + LB). The fecal weight, water content, and total gastrointestinal transit time were measured to determine whether the mice were constipated. Small bowel and colon tissue damage was assessed by hematoxylin and eosin staining, while the degree of damage was determined by double-blind scoring. The levels of serum oxidative stress markers malondialdehyde, superoxide dismutase, glutathione peroxidase, and catalase and neurotransmitters motilin, gastrin, substance P, endothelin, somatostatin, and vasoactive intestinal peptide were measured. The gene expression levels of endothelial nitric oxide synthase, inducible nitric oxide synthase, neuronal nitric oxide synthase, nuclear factor kappa-B, and cyclooxygenase-2 in small intestine tissue were calculated. The constipation symptoms of mice in H-Lop group were manifested by a variety of physiological indicators. In addition, compared with the H-Lop group, H-Lop + ZC02H could effectively relieve the symptoms of constipation in mice. In symptom characterization, the mice in the H-Lop + ZC02H group lost weight and increased feces and water content. In functional experiments, gastrointestinal motility was enhanced; the inflammation score of intestinal tissue was decreased, and gene expression levels were modulated; serum oxidative factor levels were modulated, and oxidative stress levels were decreased.
功能性便秘是最常见的胃肠道疾病之一。氧化应激可加重器官功能障碍。肠神经递质对肠神经系统和肠道肌肉收缩的调节有重要作用。氧化应激和胃肠道动力降低被认为是便秘的主要原因之一。本研究旨在探讨 Limosi 戊糖片球菌 CQZC02 是否能缓解高脂肪饮食（HFD）条件下洛哌丁胺（Lop）诱导的便秘小鼠的症状，并阐明其作用机制，重点在于肠神经递质。将 4 周龄雌性 BALB/c 小鼠随机分为 5 组：正常组（Nor）、便秘模型组（H-Lop）、戊糖片球菌 CQZC02 低剂量组（H-Lop+ZC02L）、戊糖片球菌 CQZC02 高剂量组（H-Lop+ZC02H）和保加利亚乳杆菌对照组（H-Lop+LB）。通过测量粪便重量、含水量和全胃肠道转运时间来确定小鼠是否便秘。采用苏木精和伊红染色评估小肠和结肠组织损伤，并用双盲评分法确定损伤程度。测量血清氧化应激标志物丙二醛、超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶以及神经递质胃动素、胃泌素、P 物质、内皮素、生长抑素和血管活性肠肽的水平。计算小肠组织内皮型一氧化氮合酶、诱导型一氧化氮合酶、神经元型一氧化氮合酶、核因子 kappa-B 和环氧化酶-2 的基因表达水平。
H-Lop 组小鼠出现多种生理指标的便秘症状。与 H-Lop 组相比，H-Lop+ZC02H 能有效缓解便秘症状。在症状描述中，H-Lop+ZC02H 组小鼠体重减轻，粪便和含水量增加。在功能实验中，胃肠动力增强；肠道组织炎症评分降低，基因表达水平得到调节；血清氧化因子水平得到调节，氧化应激水平降低。
