Department of Oral Mucosal Diseases, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou 350000, China.
School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350000, China.
J Tradit Chin Med. 2022 Oct;42(5):693-700. doi: 10.19852/j.cnki.jtcm.2022.05.003.
The aim of this study was to examine the antitumor effects of Qilan preparation on oral squamous cell carcinoma (OSCC) and to investigate its underlying mechanisms of action.
Cell proliferation, cell cycle distribution and apoptosis were examined using cell counting kit-8 (CCK8) and flow cytometry (FCM). The expression of PTEN and PDCD4 were determined by western blot. Changes in miR-21 levels were quantified using TaqMan stem-loop real-time PCR. After miR-21 was transiently transfected into Tca8113 cells using Lipofectamine®3000, cell proliferation, apoptosis and miR-21 and PDCD4 expression levels were measured.
Qilan preparation inhibited Tca8113 cell growth in a dose- and time-dependent manner by inducing apoptosis and cell cycle arrest in S-phase, decreasing miR-21 levels and increasing PTEN and PDCD4 expression. MiR-21 overexpression reversed the Qilan preparation-induced suppression of cell proliferation and induction of apoptosis while also blocking the increase in PDCD4.
Our study revealed, for the first time, the ability of Qilan preparation to suppress TSCC cell growth and elucidated that Qilan preparation elicits its anti-cancer actions either the miR-21/PDCD4 or PTEN pathway.
本研究旨在探讨芪兰制剂对口腔鳞状细胞癌（OSCC）的抗肿瘤作用，并探讨其作用机制。
采用细胞计数试剂盒-8（CCK8）和流式细胞术（FCM）检测细胞增殖、细胞周期分布和细胞凋亡。采用 Western blot 检测 PTEN 和 PDCD4 的表达。采用 TaqMan 茎环实时 PCR 定量检测 miR-21 水平的变化。用 Lipofectamine®3000 将 miR-21 瞬时转染 Tca8113 细胞后，检测细胞增殖、细胞凋亡以及 miR-21 和 PDCD4 的表达水平。
芪兰制剂以剂量和时间依赖的方式抑制 Tca8113 细胞生长，通过诱导细胞凋亡和 S 期细胞周期阻滞，降低 miR-21 水平，增加 PTEN 和 PDCD4 的表达。miR-21 的过表达逆转了芪兰制剂对细胞增殖的抑制和诱导的凋亡，同时阻断了 PDCD4 的增加。
本研究首次揭示了芪兰制剂抑制 TSCC 细胞生长的能力，并阐明芪兰制剂通过 miR-21/PDCD4 或 PTEN 通路发挥其抗癌作用。
