DEVELOPMENT OF IN-SITU SYSTEMS FOR 13CO2 EXPOSURE AND DETERMINATION OF 13C NET ASSIMILATION RATE OF FRUIT-BEARING SHOOTS AND WHOLE TREE AND CHAMBER FOR PRECISION 13CO2 EXPOSURE OF YOUNG POTTED TREES.

14C is the most important radionuclide for assessing exposure dose around the Rokkasho nuclear fuel reprocessing plant, Aomori Prefecture, Japan. A simplified model with ample margins has been used so far for the assessment of the dose derived from 14C. Realistic dose estimate of 14C using more realistic model is necessary for safety. Apple production is an important core industry in Aomori Prefecture. To construct the dynamic model for apple, using 13C as a tracer to substitute for 14C, we collected data on 13C abundance in organs including fruit after 13CO2 exposure at various fruit growth stages. We developed 13CO2 exposure systems for three intact fruit-bearing shoots (1), whole mature tree (2) and young potted trees (3). Systems (1) and (2) also can determine net amounts of carbon and 13C photoassimilated. System (3) is capable of precise feedback control of 13CO2 and 12CO2 concentrations based on on-time determination.