Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou, Henan, PR China.
Guangdong Provincial Engineering Technology Research Center of Environmental and Health Risk Assessment, Department of Occupational and Environmental Health, School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong, PR China.
Environ Int. 2022 Nov;169:107496. doi: 10.1016/j.envint.2022.107496. Epub 2022 Sep 5.
Both ambient ozone exposure and residential greenness are linked to the aging process. However, their interactive effect on molecular biomarkers of aging (telomere length (TL) and mitochondrial DNA copy number (mtDNA-CN)) remains unclear.
This study was conducted among 6418 rural Chinese adults. The concentration of ambient ozone was assessed using a random forest model. Residential greenness was represented by the normalized difference vegetation index (NDVI). Molecular biomarkers of aging (relative TL and relative mtDNA-CN) were determined by quantitative real-time polymerase chain reaction. Generalized linear regression models were applied to investigate the independent and combined effects of ambient ozone and residential greenness on relative TL and relative mtDNA-CN.
The estimated percent changes and 95 % confidence intervals (CIs) of relative TL in response to per-unit increase in ambient ozone were -22.43 % (-23.74 %, -21.18 %), -14.19 % (-15.63 %, -12.72 %) and -4.50 % (-6.57 %, -2.27 %) for participants with low (NDVI ≤ 0.53), moderate (0.54-0.55) and high (≥0.56) residential greenness exposure, respectively, while the corresponding figures of relative mtDNA-CN were -12.63 % (-13.84 %, -11.31 %), -9.52 % (-10.60 %, -8.33 %) and 2.12 % (0.20 %, 4.19 %). Furthermore, negative interactive effects between ambient ozone and residential greenness exposure on molecular biomarkers of aging were observed (P < 0.001 for relative TL, and 0.098 for relative mtDNA-CN).
Long-term exposure to high concentrations of ambient ozone and low residential greenness was associated with decreased mtDNA-CN and shortened TL. The adverse effect of ambient ozone exposure on molecular biomarkers of aging may be attenuated by increased residential greenness.
环境臭氧暴露和居住绿化都与衰老过程有关。然而，它们对衰老分子生物标志物（端粒长度（TL）和线粒体 DNA 拷贝数（mtDNA-CN））的相互作用影响尚不清楚。
本研究在中国农村 6418 名成年人中进行。使用随机森林模型评估环境臭氧浓度。居住绿化用归一化差异植被指数（NDVI）表示。通过实时定量聚合酶链反应测定衰老的分子生物标志物（相对 TL 和相对 mtDNA-CN）。应用广义线性回归模型研究环境臭氧和居住绿化对相对 TL 和相对 mtDNA-CN 的独立和联合作用。
环境臭氧每增加一个单位，相对 TL 响应的估计百分比变化和 95%置信区间（CI）分别为-22.43%（-23.74%，-21.18%）、-14.19%（-15.63%，-12.72%）和-4.50%（-6.57%，-2.27%），分别为居住绿化暴露低（NDVI≤0.53）、中（0.54-0.55）和高（≥0.56）的参与者，而相对 mtDNA-CN 的相应值分别为-12.63%（-13.84%，-11.31%）、-9.52%（-10.60%，-8.33%）和 2.12%（0.20%，4.19%）。此外，还观察到环境臭氧和居住绿化暴露对衰老分子生物标志物的负交互作用（相对 TL 为 P<0.001，相对 mtDNA-CN 为 0.098）。
长期暴露于高浓度环境臭氧和低居住绿化与 mtDNA-CN 减少和 TL 缩短有关。居住绿化增加可能会减轻环境臭氧暴露对衰老分子生物标志物的不良影响。
