Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China; Department of Neurosurgery, Emergency Medicine Center, Zhejiang Provincial People's Hospital, Hangzhou Medical College, Hangzhou, Zhejiang, China; The Key Laboratory of Myocardial Ischemia, Ministry of Education, Heilongjiang Province, Harbin, China.
The Key Laboratory of Myocardial Ischemia, Ministry of Education, Heilongjiang Province, Harbin, China; Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Thromb Res. 2022 Nov;219:1-13. doi: 10.1016/j.thromres.2022.08.024. Epub 2022 Aug 27.
Neutrophil extracellular traps (NETs) are known to play a role in various diseases affecting coagulation. As of now, it is unclear whether NETs are present in hematoma samples collected from patients who have suffered an intracranial hemorrhage (ICH). The objective of this was to determine whether NETs are present in circulation and hematoma samples from ICH patients and to evaluate the procoagulant activity (PCA) of NETs during the ICH process. The expression of NET markers in samples from 78 ICH patients and 35 healthy donners was detected by ELISA and flow cytometry. Immunostaining for neutrophil markers (neutrophil CD66b) and NET markers (citrullinated histone H3 [H3Cit] and extracellular DNA) was performed on hematoma samples obtained from ICH patients undergoing intracranial hematoma evacuation. Our findings suggest that plasma and hematoma samples from patients with ICH showed high levels of NETs. Furthermore, using DNase I to target NETs enhanced ex vivo hematoma lysis. In conclusion, NETs play an important role in the ICH process and may be a novel therapeutic target for treatment of ICH patients.
中性粒细胞胞外诱捕网（NETs）在影响凝血的各种疾病中发挥作用。到目前为止，尚不清楚 NETs 是否存在于颅内出血（ICH）患者的血肿样本中。本研究旨在确定 NETs 是否存在于 ICH 患者的循环和血肿样本中，并评估 NETs 在 ICH 过程中的促凝活性（PCA）。通过 ELISA 和流式细胞术检测了 78 例 ICH 患者和 35 名健康供体样本中 NET 标志物的表达。对接受颅内血肿清除术的 ICH 患者的血肿样本进行了中性粒细胞标志物（中性粒细胞 CD66b）和 NET 标志物（瓜氨酸化组蛋白 H3 [H3Cit]和细胞外 DNA）的免疫染色。我们的研究结果表明，ICH 患者的血浆和血肿样本显示出高水平的 NETs。此外，使用 DNAse I 靶向 NETs 可增强体外血肿溶解。总之，NETs 在 ICH 过程中起重要作用，可能成为治疗 ICH 患者的新的治疗靶点。
