Department of Chemistry, City University of Hong Kong, Hong Kong SAR, China.
Key Laboratory of Environmental Pollution and Integrative Omics, Guilin Medical University, Education Department of Guangxi Zhuang Autonomous Region, China; Department of Chemistry, City University of Hong Kong, Hong Kong SAR, China; State Key Laboratory of Marine Pollution, City University of Hong Kong, Hong Kong SAR, China.
Mar Pollut Bull. 2022 Oct;183:114093. doi: 10.1016/j.marpolbul.2022.114093. Epub 2022 Sep 7.
17α-ethinylestradiol (EE2) is an anthropogenic estrogen that is widely used for hormone therapy and oral contraceptives. It was reported that EE2 exposure induced reproductive impairments through processes affecting reproduction behavior and inducing ovotestis. However, the effects of continuous EE2 exposure on the reproductive performance remain largely unknown. In this study, adult marine medaka fish (Oryzias melastigma) were exposed to EE2 (85 ng/L) for one (F) and two (F) generations. Our results indicate that continuous EE2 exposure reduced fecundity and sperm motility. The testicular transcriptome, followed by bioinformatic analysis revealed the dysregulation of pathways related to steroidogenesis, sperm motility, and reproductive system development. Collectively, our findings indicate that continuous EE2 exposure directly affected sperm quality via the alteration of steroidogenesis and dysregulation of reproductive system development. The identified key factors including DNM1, PINK1, PDE7B, and SLC12A7 can serve as biomarkers to assess EE2-reduced sperm motility.
17α-乙炔基雌二醇（EE2）是一种人为雌激素，广泛用于激素治疗和口服避孕药。据报道，EE2 暴露通过影响生殖行为和诱导卵睾的过程导致生殖损伤。然而，连续 EE2 暴露对生殖性能的影响在很大程度上仍然未知。在这项研究中，成年海洋稻鱼（Oryzias melastigma）被暴露于 EE2（85ng/L）一个（F）和两个（F）代。我们的结果表明，连续 EE2 暴露降低了生育力和精子活力。睾丸转录组，接着是生物信息学分析揭示了与类固醇生成、精子活力和生殖系统发育相关的途径的失调。总的来说，我们的研究结果表明，连续 EE2 暴露通过改变类固醇生成和生殖系统发育的失调直接影响精子质量。鉴定的关键因素包括 DNM1、PINK1、PDE7B 和 SLC12A7 可以作为评估 EE2 降低精子活力的生物标志物。
Zotero 插件