Lee Po-Yen, Lin Chun-Yao, Chen Te-Hao
Institute of Marine Biodiversity and Evolutionary Biology, National Dong Hwa University, Pingtung, Taiwan.
Department of Planning and Research, National Museum of Marine Biology and Aquarium, Pingtung, Taiwan.
Mar Pollut Bull. 2014 Aug 30;85(2):338-43. doi: 10.1016/j.marpolbul.2014.04.013. Epub 2014 Apr 26.
We assessed the effects of 17α-ethinylestradiol (EE2) on spawning and reproductive behavior of the brackish medaka Oryzias melastigma at environmental concentrations. Breeding pairs were exposed to EE2 at either lower concentrations (0, 1, and 10 ng/L) or higher concentrations (0, 50, and 100 ng/L) for 14 days. Swimming performance and reproductive behavior (following, dancing, and copulation) of the males were analyzed at the end of exposure. Additionally, gonad histology was conducted in the males in the higher EE2 experiment. We found that spawning was significantly inhibited in the 50-100 ng/L EE2 groups. Swimming performance was not affected in all groups. Dancing and copulation were significantly suppressed in the 50-100 ng/L groups. No effect was observed in testis histology in the 50-100 ng/L groups. Our data suggests that reproductive behavior of O. melastigma can be utilized as a behavioral endpoint for ecotoxicology studies of endocrine disruptors in estuarine/marine environments.
我们评估了环境浓度下17α-乙炔雌二醇（EE2）对半咸水青鳉（Oryzias melastigma）产卵和繁殖行为的影响。将繁殖对暴露于较低浓度（0、1和10纳克/升）或较高浓度（0、50和100纳克/升）的EE2中14天。在暴露结束时分析雄性的游泳性能和繁殖行为（跟随、跳舞和交配）。此外，在较高EE2浓度实验中对雄性进行了性腺组织学检查。我们发现，50-100纳克/升EE2组的产卵受到显著抑制。所有组的游泳性能均未受影响。50-100纳克/升组的跳舞和交配行为受到显著抑制。在50-100纳克/升组中，未观察到睾丸组织学有影响。我们的数据表明，半咸水青鳉的繁殖行为可作为河口/海洋环境中内分泌干扰物生态毒理学研究的行为终点。
