School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, China.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
Fish Shellfish Immunol. 2022 Nov;130:233-243. doi: 10.1016/j.fsi.2022.09.004. Epub 2022 Sep 7.
Long non-coding RNA (lncRNA) play vital regulatory roles in various biological processes. Intestine is one of the most sensitive organs to environmental and homeostatic disruptions for fish. However, systematic profiles of lncRNAs in the intestine of teleost in responses to pathogen infections is still limited. Turbot (Scophthalmus maximus L.), an important commercial fish species in China, has been suffering with Vibrio anguillarum infection, resulted in dramatic economic loss. Hereinto, the intestinal tissues of turbot were sampled at 0 h, 2 h, 12 h, and 48 h following V. anguillarum infection. The histopathological analysis revealed that the pathological trauma was mainly present in intestinal tunica mucosal epithelium. After high-throughput sequencing and bioinformatic analysis, a total of 9722 lncRNAs and 21,194 mRNAs were obtained, and the average length and exon number of lncRNAs were both less than those of mRNAs. Among which, a set of 158 lncRNAs and 226 mRNAs were differentially expressed (DE-lncRNAs and DEGs) in turbot intestine at three time points, related to many immune-related genes such as complement, interleukin, chemokine, lysosome, and macrophage, indicating their potential critical roles in immune responses. In addition, 2803 and 1803 GO terms were enriched for DEGs and co-expressed target genes of DE-lncRNAs, respectively. Moreover, 127 and 50 KEGG pathways including cell adhesion molecules (CAMs), phagosome, JAK-STAT signaling pathway, cytokine-cytokine receptor interaction, and intestinal immune network for IgA production, were enriched for DEGs and co-expressed target genes of DE-lncRNAs, respectively. Finally, qRT-PCR was conducted to confirm the reliability of sequencing data. The present study will set the foundation for the future exploration of lncRNA functions in teleost in response to bacterial infection.
长链非编码 RNA（lncRNA）在各种生物过程中发挥着重要的调节作用。肠道是鱼类对环境和内稳态紊乱最敏感的器官之一。然而，系统的长链非编码 RNA 谱在鱼类肠道对病原体感染的反应中仍然有限。大菱鲆（Scophthalmus maximus L.）是中国重要的商业鱼类品种，一直受到鳗弧菌感染的困扰，造成了巨大的经济损失。在本研究中，在鳗弧菌感染后 0 h、2 h、12 h 和 48 h 分别采集大菱鲆的肠道组织。组织病理学分析表明，病变主要存在于肠道黏膜上皮。经过高通量测序和生物信息学分析，共获得 9722 个 lncRNA 和 21194 个 mRNA，lncRNA 的平均长度和外显子数均小于 mRNA。其中，在三个时间点大菱鲆肠道中有一组 158 个 lncRNA 和 226 个 mRNAs 差异表达（DE-lncRNAs 和 DEGs），与补体、白细胞介素、趋化因子、溶酶体和巨噬细胞等许多免疫相关基因相关，表明它们在免疫反应中具有潜在的关键作用。此外，DEGs 和 DE-lncRNAs 共表达靶基因分别富集了 2803 和 1803 个 GO 术语。此外，127 和 50 个 KEGG 通路包括细胞黏附分子（CAMs）、吞噬体、JAK-STAT 信号通路、细胞因子-细胞因子受体相互作用和 IgA 产生的肠道免疫网络，分别富集了 DEGs 和 DE-lncRNAs 共表达靶基因。最后，进行了 qRT-PCR 以验证测序数据的可靠性。本研究将为未来探索长链非编码 RNA 在鱼类对细菌感染的反应中的功能奠定基础。
Zotero 插件