Department of Neurosurgery, Neurosurgery Research Institute, The First Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian, China.
Jianning County General hospital, Sanming, Fujian, China.
Commun Biol. 2022 Sep 9;5(1):941. doi: 10.1038/s42003-022-03900-0.
Tumefactive demyelinating lesion (TDL) is an immune-mediated disease which can be misdiagnosed as glioma. At present, there is no study comparing difference between the two disorders at the cellular level. Here, we perform integrative and comparative single-cell RNA sequencing (ScRNA-seq) transcriptomic analysis on TDL and glioma lesions. At single-cell resolution, TDL is comprised primarily of immune cells, which is completely different from glioma. The integrated analysis reveals a TDL-specific microglial subset involving in B cell activation and proliferation. Comparative analysis highlights remyelination function of glial cells and demyelination function of T cells in TDL. Subclustering and pseudotime trajectory analysis of T cells in TDL reveal their heterogeneity and diverse functions involving in TDL pathogenesis and recovery process. Our study identifies substantial differences between TDL and glioma at single-cell resolution. The observed heterogeneity and potentially diverse functions of cells in TDL may be critical in disease progression.
肿块样脱髓鞘病变（TDL）是一种免疫介导的疾病，可能被误诊为神经胶质瘤。目前，尚无研究在细胞水平上比较这两种疾病的差异。在这里，我们对 TDL 和神经胶质瘤病变进行了综合和比较的单细胞 RNA 测序（ScRNA-seq）转录组分析。在单细胞分辨率下，TDL 主要由免疫细胞组成，这与神经胶质瘤完全不同。整合分析揭示了 TDL 中涉及 B 细胞激活和增殖的特定小胶质细胞亚群。比较分析突出了 TDL 中神经胶质细胞的髓鞘再生功能和 T 细胞的脱髓鞘功能。TDL 中 T 细胞的亚群聚类和拟时轨迹分析揭示了它们的异质性和多种功能，这些功能涉及 TDL 的发病机制和恢复过程。我们的研究在单细胞分辨率上确定了 TDL 和神经胶质瘤之间的显著差异。TDL 中细胞的观察到的异质性和潜在的多种功能可能在疾病进展中起关键作用。
