Fragoso-Fonseca David Esaú, Ruiz-Hernández Ubaldo Emilio, Trujillo-Salgado Brenda Berenice, Manuell-Barrios Rita Teresita, Garcés-Ayala Fabiola, Del Mazo-López Juan Carlos, Méndez-Tenorio Alfonso, Hernández-Rivas Lucía, Ramírez-González José Ernesto, Escobar-Escamilla Noé
Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) "Dr. Manuel Martínez Báez", Secretaría de Salud, Mexico City, Mexico.
Laboratorio de Bioinformática Genómica, Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional (IPN), Mexico City, Mexico.
Arch Virol. 2022 Dec;167(12):2795-2800. doi: 10.1007/s00705-022-05589-2. Epub 2022 Sep 10.
Human papillomavirus 31 (HPV31) is the fourth most frequent high-risk HPV (HR-HPV) genotype identified in cervical cancer (CC) worldwide and in Mexico. It has been recently classified into three lineages (A, B, and C) and eight sublineages (A1, A2, B1, B2, and C1 - C4). Here, we report the complete genomic sequences of 14 HPV31 isolates from cervical samples, and these were compared with viral genome sequences from the GenBank database for phylogenetic and genetic distance analysis. The formation of two novel clades within the C lineage (proposed as C5 and C6) was observed, with a well-defined variant-specific mutational pattern. The smallest average pairwise distance was 0.71% for lineages A and B, 0.94% for lineages A and C, and 1.01% for lineages B and C, and between sublineages, these values were 0.21% for clade A, 0.29% for clade B, and 0.24% for clade C. The isolates were grouped into the sublineages A1, B2, C1-C3, and C6. This is the first report on the whole-genome diversity of HPV31 in Mexico.
人乳头瘤病毒31型（HPV31）是在全球范围内以及墨西哥宫颈癌（CC）中鉴定出的第四常见的高危人乳头瘤病毒（HR-HPV）基因型。最近它被分为三个谱系（A、B和C）和八个亚谱系（A1、A2、B1、B2以及C1 - C4）。在此，我们报告了来自宫颈样本的14株HPV31分离株的完整基因组序列，并将这些序列与来自GenBank数据库的病毒基因组序列进行比较，以进行系统发育和遗传距离分析。在C谱系中观察到形成了两个新的进化枝（提议命名为C5和C6），具有明确的变异特异性突变模式。A和B谱系之间的最小平均成对距离为0.71%，A和C谱系之间为0.94%，B和C谱系之间为1.01%，而在亚谱系之间，A进化枝的值为0.21%，B进化枝为0.29%，C进化枝为0.24%。这些分离株被归入亚谱系A1、B2、C1 - C3和C6。这是墨西哥关于HPV31全基因组多样性的首次报告。
