Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Periodontics, School of Dentistry, Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Photochem Photobiol. 2023 May-Jun;99(3):1003-1009. doi: 10.1111/php.13711. Epub 2022 Sep 29.
Gingival fibroblasts have critical roles in oral wound healing. Photobiomodulation (PBM) has been shown to promote mucosal healing and is now recommended for managing oncotherapy-associated oral mucositis. This study examined the effects of the emission mode of a 940 nm diode laser on the viability and migration of human gingival fibroblasts. Cells were cultured in a routine growth media and treated with PBM (average power 0.1 W cm , average fluence 3 J cm , every 12 h for six sessions) in one continuous wave and two pulsing settings with 20% and 50% duty cycles. Cell viability was assessed using MTT, and digital imaging quantified cell migration. After 48 and 72 h, all treatment groups had significantly higher viability (n = 6, P < 0.05) compared with the control. The highest viability was seen in the pulsed (20% duty cycle) group at the 72-h time point. PBM improved fibroblast migration in all PBM-treated groups, but differences were not statistically significant (n = 2, P > 0.05). PBM treatments can promote cell viability in both continuous and pulsed modes. Further studies are needed to elucidate the optimal setting for PBM-evoked responses for its rationalized use in promoting specific phases of oral wound healing.
牙龈成纤维细胞在口腔伤口愈合中起着关键作用。光生物调节（PBM）已被证明可促进黏膜愈合，目前被推荐用于治疗与癌症治疗相关的口腔粘膜炎。本研究探讨了 940nm 二极管激光发射模式对人牙龈成纤维细胞活力和迁移的影响。细胞在常规生长培养基中培养，并采用连续波和两种占空比为 20%和 50%的脉冲设置，以平均功率 0.1W/cm2 和平均辐照度 3J/cm2 进行 PBM（每 12 小时一次，共进行六次）处理。使用 MTT 评估细胞活力，数字成像定量细胞迁移。在 48 和 72 小时后，与对照组相比，所有治疗组的细胞活力均显著升高（n=6，P<0.05）。在 72 小时时，以脉冲（20%占空比）组的细胞活力最高。PBM 可促进所有 PBM 处理组的成纤维细胞迁移，但差异无统计学意义（n=2，P>0.05）。PBM 治疗可以在连续和脉冲模式下促进细胞活力。需要进一步的研究来阐明 PBM 激发反应的最佳设置，以便合理地用于促进口腔伤口愈合的特定阶段。
