染色质相关的微处理器组装受到 U1 snRNP 辅助蛋白 PRP40 的调控。
Chromatin-associated microprocessor assembly is regulated by the U1 snRNP auxiliary protein PRP40.
机构信息
Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznan 61-614, Poland.
Department of Cellular and Molecular Biology, Nicolaus Copernicus University, Torun 87-100, Poland.
出版信息
Plant Cell. 2022 Nov 29;34(12):4920-4935. doi: 10.1093/plcell/koac278.
In plants, microRNA (miRNA) biogenesis involves cotranscriptional processing of RNA polymerase II (RNAPII)-generated primary transcripts by a multi-protein complex termed the microprocessor. Here, we report that Arabidopsis (Arabidopsis thaliana) PRE-MRNA PROCESSING PROTEIN 40 (PRP40), the U1 snRNP auxiliary protein, positively regulates the recruitment of SERRATE, a core component of the plant microprocessor, to miRNA genes. The association of DICER-LIKE1 (DCL1), the microprocessor endoribonuclease, with chromatin was altered in prp40ab mutant plants. Impaired cotranscriptional microprocessor assembly was accompanied by RNAPII accumulation at miRNA genes and retention of miRNA precursors at their transcription sites in the prp40ab mutant plants. We show that cotranscriptional microprocessor assembly, regulated by AtPRP40, positively affects RNAPII transcription of miRNA genes and is important to reach the correct levels of produced miRNAs.
在植物中，microRNA (miRNA) 的生物发生涉及 RNA 聚合酶 II (RNAPII) 生成的初级转录物的共转录加工，由称为微处理器的多蛋白复合物完成。在这里，我们报告拟南芥 (Arabidopsis thaliana) 的 PRE-MRNA PROCESSING PROTEIN 40 (PRP40)，即 U1 snRNP 辅助蛋白，正向调节 SERRATE 的募集，SERRATE 是植物微处理器的核心组成部分，到 miRNA 基因。DICER-LIKE1 (DCL1)，即微处理器内切核酸酶，与染色质的结合在 prp40ab 突变体植物中发生改变。共转录微处理器组装受损伴随着 miRNA 基因处的 RNAPII 积累和 miRNA 前体在其转录位点的保留，在 prp40ab 突变体植物中。我们表明，由 AtPRP40 调控的共转录微处理器组装，对 miRNA 基因的 RNAPII 转录具有正向影响，对于达到产生的 miRNA 的正确水平非常重要。
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