通过化学蛋白质组学对铁死亡中的蛋白质羰基化进行分析。
Profiling of Protein Carbonylations in Ferroptosis by Chemical Proteomics.
机构信息
Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.
Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China.
出版信息
Methods Mol Biol. 2022;2543:141-153. doi: 10.1007/978-1-0716-2553-8_12.
Ferroptosis is a new form of cell death with hallmark of lipid peroxidation and iron accumulation. It has been shown that lipid peroxidation can result in electrophilic metabolites which in turn induce protein carbonylations. Identification of specific carbonylated proteins and sites in ferroptotic cells will be of great significance for understanding the mechanism and discovering potential biomarkers for this new cell death. The protocol described herein is an optimized pipeline which combines the labeling of carbonylated proteins by a commercially available aniline-based probe with the tandem orthogonal proteolysis activity-based protein profiling (TOP-ABPP) strategy to portrait the landscape of carbonylations in ferroptotic cells.
铁死亡是一种新的细胞死亡形式，具有脂质过氧化和铁积累的特征。已经表明，脂质过氧化可以导致亲电代谢物，进而诱导蛋白质羰基化。在铁死亡细胞中鉴定特定的羰基化蛋白质和位点对于理解该新的细胞死亡机制和发现潜在的生物标志物将具有重要意义。本文所述的方案是一种优化的管道，它结合了一种商业上可用的基于苯胺的探针对羰基化蛋白的标记与串联正交蛋白水解活性的基于蛋白质谱（TOP-ABPP）策略，以描绘铁死亡细胞中羰基化的全景。
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