腺样囊性癌中线粒体动态标志物的表达。

Expression of mitochondrial dynamic markers in adenoid cystic carcinoma.

机构信息

Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

Department of Oral Diagnosis, Area of Pathology, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil.

出版信息

J Oral Pathol Med. 2022 Sep;51(8):702-709. doi: 10.1111/jop.13340. Epub 2022 Aug 17.

DOI:10.1111/jop.13340

PMID:36087273

Abstract

BACKGROUND

Mitochondrial fission and fusion processes are known as mitochondrial dynamics and the occurrence of imbalances in the mitochondrial activity is related to the pathogenesis of many human cancers. However, the importance of mitochondrial dynamics in malignant salivary gland tumours remains unknown. Therefore, we aimed to investigate its prognostic significance in adenoid cystic carcinoma.

METHODS

Fifty-seven formalin-fixed paraffin-embedded cases were retrieved and disposed in tissue microarray. Histological sections were submitted to immunohistochemical reactions against AMT, DRP1, FIS1, MFN1, MFN2 and OPA1 proteins. Clinical data were retrieved from the patients' medical files, including specific and disease-free survival data.

RESULTS

It was observed that 50.9% of the cases were strongly positive for AMT and DRP1, and 49.1%, 21.1%, 22.8% and 24.6% strongly positive for FIS1, MFN1, MFN2 and OPA1, respectively. Reactions were observed in both epithelial and myoepithelial components of the tumour. The higher expression of MFN2 was associated with solid microscopic pattern (p = 0.016). DRP1 overexpression showed a trend towards a shorter overall survival (p = 0.054), while negative/weak OPA1 showed a trend towards a lower disease-free survival (p = 0.051) in the univariate analysis, but no mitochondrial marker represented an independent prognostic determinant under multivariate analysis.

CONCLUSION

In conclusion, mitochondrial dynamics markers do not seem to carry a prognostic significance for adenoid cystic carcinoma patients, but these proteins may play an important role in its pathogenesis.

摘要

背景

线粒体的分裂和融合过程被称为线粒体动力学，线粒体活性的失衡与许多人类癌症的发病机制有关。然而，线粒体动力学在恶性涎腺肿瘤中的重要性尚不清楚。因此，我们旨在研究其在腺样囊性癌中的预后意义。

方法

检索并处理了 57 例福尔马林固定石蜡包埋的病例，制作组织微阵列。对 AMT、DRP1、FIS1、MFN1、MFN2 和 OPA1 蛋白进行组织切片的免疫组织化学反应。从患者的病历中检索临床数据，包括特定和无病生存数据。

结果

观察到 50.9%的病例 AMT 和 DRP1 强阳性，49.1%、21.1%、22.8%和 24.6%的病例 FIS1、MFN1、MFN2 和 OPA1 强阳性。反应发生在肿瘤的上皮和肌上皮成分中。MFN2 的高表达与实体微观模式相关（p=0.016）。DRP1 过表达在单因素分析中显示出总生存期较短的趋势（p=0.054），而 OPA1 阴性/弱阳性显示出无病生存期较低的趋势（p=0.051），但在多因素分析中，没有线粒体标志物是独立的预后决定因素。