Diel de Amorim Mariana, Bramer Sarah A, Rajamanickam Gayathri D, Klein Claudia, Card Claire
Department of Clinical Sciences, Cornell University, 930 Campus Rd, Ithaca, NY 14853, USA.
Department of Veterinary Clinical and Diagnostic Science, University of Calgary, 2500 University Drive NW, Calgary, Alberta T2N 1N4, Canada.
Anim Reprod Sci. 2022 Oct;245:107064. doi: 10.1016/j.anireprosci.2022.107064. Epub 2022 Sep 1.
Our understanding of the temporal changes in endometrial and luteal gene transcripts related to the actions of oxytocin and prostaglandin during early equine pregnancy is incomplete. Additionally, the role of oxytocinase, also known as Leucyl-cystinyl aminopeptidase (LNPEP), during early pregnancy in mares has not been previously investigated. Luteal and endometrial biopsies were obtained on Day (D)8, D10, D12 and D15 post-ovulation in pregnant (PREG) and diestrus (DIEST) mares for real-time qPCR. Differences in endometrial gene expression occurred over time in: SLC2A4, SLC2A1, PTGES, OXTR and LNPEP. PTGFR and PLA2G2C had lower relative abundance in PREG D15 endometrium compared to D10. OXT and OXTR were increased on D10 and 15 PREG, respectively. Regarding luteal mRNA relative abundance, ESR1, PTGS2, PTGFR, and PTGES had higher relative abundance in D12 of DIEST and PREG. Luteal expression of OXTR and OXT had higher relative abundance in D15 compared to D8, and LNPEP had higher relative abundance in D10 and 12. Endometrial and luteal PTGES had an increased mRNA abundance in both D12 DIEST and PREG mares, which may lead to additional luteoprotective prostaglandin E2 (PGE2) secretion. Furthermore, luteal SLC2A1 had higher relative abundance in pregnancy, and likely supports the high metabolic activity of luteal tissue by increasing glucose uptake. Oxytocinase is present in endometrial and luteal tissue and its role in oxytocin induced prostaglandin secretion is uncertain.
我们对马早期妊娠期间与催产素和前列腺素作用相关的子宫内膜和黄体基因转录本的时间变化的理解并不完整。此外，催产素酶，也称为亮氨酰 - 胱氨酰氨基肽酶（LNPEP），在母马早期妊娠中的作用此前尚未得到研究。在排卵后第8天（D8）、第10天（D10）、第12天（D12）和第15天（D15），从怀孕（PREG）和发情间期（DIEST）的母马获取黄体和子宫内膜活检组织用于实时定量PCR。子宫内膜基因表达的差异在以下基因中随时间出现：SLC2A4、SLC2A1、PTGES、OXTR和LNPEP。与D10相比，PTGFR和PLA2G2C在PREG D15子宫内膜中的相对丰度较低。OXT和OXTR分别在PREG的第10天和第15天增加。关于黄体mRNA相对丰度，ESR1、PTGS2、PTGFR和PTGES在DIEST和PREG的D12中具有较高的相对丰度。与D8相比，OXTR和OXT在黄体中的表达在D15时相对丰度更高，而LNPEP在D10和D12时相对丰度更高。在DIEST和PREG的D12母马中，子宫内膜和黄体中的PTGES的mRNA丰度均增加，这可能导致额外的黄体保护前列腺素E2（PGE2）分泌。此外，黄体中的SLC2A1在妊娠时相对丰度更高，可能通过增加葡萄糖摄取来支持黄体组织的高代谢活性。催产素酶存在于子宫内膜和黄体组织中，其在催产素诱导的前列腺素分泌中的作用尚不确定。
