Department of Biotechnology, College of Convergence Engineering, Sangmyung University, Seoul 03016, Republic of Korea.
Department of Marine Science, College of Natural Sciences, Incheon National University, Incheon 22012, Republic of Korea.
Comp Biochem Physiol C Toxicol Pharmacol. 2022 Dec;262:109462. doi: 10.1016/j.cbpc.2022.109462. Epub 2022 Sep 7.
Owing to its high production and world-wide usage, plastic pollution is an increasing concern in marine environments. Plastic is decomposed into nano- and micro-sized debris, which negative affect reproduction and development in aquatic organisms. Bisphenol A (BPA), an additive of plastic, is released into the water column upon plastic degradation, and is known as a representative endocrine-disrupting chemical. However, the reproductive effects of plastics and bisphenols at the molecular level have not yet been explored in small marine crustaceans. In this study, we investigated the effects of polystyrene (PS) beads (0.05, 0.5, and 6 - μm) and bisphenol analogues (BPs; BPA, BPS, and BPF) on reproduction and development of small marine crustaceans. Effects on transcriptional changes in ecdysteroid and juvenile hormone (JH) signaling pathway-related genes were examined in the brackish water flea Diaphanosoma celebensis exposed to PS beads and BPs for 48 h. As results, BPs and PS beads delayed emergence time of first offspring, and increased fecundity in a concentration-dependent manner. BPs differentially modulated the expression of ecdysteroid and JH signaling pathway-related genes, indicating that BP analogs can disrupt endocrine systems via mechanisms different from those of BPA. PS beads was also changed the gene expression of both pathway, depending on their size and concentration. Our findings suggest that BP analogues and PS beads disrupt the endocrine system by modulating the hormonal pathways, affecting reproduction negatively. This study provides a better understanding of the molecular mode of action of BPs and PS beads in the reproduction of small crustaceans.
由于其高产量和全球使用率，塑料污染是海洋环境中日益令人关注的问题。塑料分解成纳米和微尺寸的碎片，对水生生物的繁殖和发育产生负面影响。双酚 A（BPA）是塑料的一种添加剂，在塑料降解时会释放到水柱中，被认为是一种代表性的内分泌干扰化学物质。然而，在小型海洋甲壳类动物中，塑料和双酚类物质在分子水平上的生殖效应尚未得到探索。在这项研究中，我们研究了聚苯乙烯（PS）珠（0.05、0.5 和 6 μm）和双酚类似物（BPs；BPA、BPS 和 BPF）对小型海洋甲壳类动物繁殖和发育的影响。在暴露于 PS 珠和 BPs 48 小时后，检查了卤淡水溞（Diaphanosoma celebensis）中蜕皮激素和保幼激素（JH）信号通路相关基因转录变化的影响。结果表明，BPs 和 PS 珠以浓度依赖的方式延迟了第一代后代的出现时间，并增加了繁殖力。BPs 差异调节了蜕皮激素和 JH 信号通路相关基因的表达，表明 BP 类似物可以通过与 BPA 不同的机制破坏内分泌系统。PS 珠也根据其大小和浓度改变了两个途径的基因表达。我们的研究结果表明，BP 类似物和 PS 珠通过调节激素途径破坏内分泌系统，对繁殖产生负面影响。本研究为了解 BPs 和 PS 珠在小型甲壳类动物繁殖中的分子作用模式提供了更好的认识。
