Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Nutrition and Food Security Research Center, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran.
Carbohydr Polym. 2022 Nov 15;296:119966. doi: 10.1016/j.carbpol.2022.119966. Epub 2022 Aug 9.
The incorporation of carbohydrate polymers is one of the most efficient strategies to reinforce protein matrices for electrospinning application. In the present work, a basil seed gum (BSG)-reinforced whey protein isolate (WPI) was developed via electrospinning for the co-encapsulation of zinc oxide nanoparticles (ZnONPs) and curcumin (CU). The physicochemical attributes of the nanofiber samples could be controlled by varying the BSG mixing ratio. The Field emission scanning electron microscopy images showed bead-free morphology of WPI/BSG/ZnONPs/CU nanofibers with average fiber diameter of around 362 ± 41 nm. The formation of new H bonds after introduction of BSG and active components was corroborated by Fourier-transform infrared spectroscopy. The nanofibers loaded with ZnONPs/CU displayed improved surface hydrophobicity and high potential for hampering colon cancer cells in vitro. The results proved that the proposed electrospun structures were thermally stable and composed by homogenous nanofibers of high bactericide properties, thus representing promising structures suitable for various biomedical applications.
碳水化合物聚合物的掺入是增强用于静电纺丝应用的蛋白质基质的最有效策略之一。在本工作中，通过静电纺丝开发了一种罗勒籽胶（BSG）增强乳清蛋白分离物（WPI），用于共包封氧化锌纳米粒子（ZnONPs）和姜黄素（CU）。通过改变 BSG 混合比可以控制纳米纤维样品的物理化学性质。场发射扫描电子显微镜图像显示 WPI/BSG/ZnONPs/CU 纳米纤维具有无珠状形态，平均纤维直径约为 362±41nm。傅里叶变换红外光谱证实了引入 BSG 和活性成分后形成了新的氢键。负载 ZnONPs/CU 的纳米纤维表现出改善的表面疏水性和体外抑制结肠癌细胞的高潜力。结果证明，所提出的静电纺丝结构具有热稳定性，并且由具有高杀菌性能的均匀纳米纤维组成，因此代表了适合各种生物医学应用的有前途的结构。
