Bui Duong T, Kitova Elena N, Mahal Lara K, Klassen John S
Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada. Electronic address: https://twitter.com/@Duong_T_Bui.
Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada.
Curr Opin Struct Biol. 2022 Dec;77:102448. doi: 10.1016/j.sbi.2022.102448. Epub 2022 Sep 8.
The non-covalent associations of complex carbohydrates (glycans) with glycan-binding proteins mediate many important physiological and pathophysiological processes. Identifying these interactions is essential to understanding their diverse biological functions and enables the development of new disease treatments and diagnostics. Knowledge of the repertoire of glycans recognized by most glycan-binding proteins and their affinities is incomplete. Mass spectrometry-based screening of natural glycan libraries has emerged as a promising approach to defining the glycan interactome of glycan-binding proteins. Here, we review recent advances in mass spectrometry-based natural library screening that have led to the discovery of glycan ligands of endogenous and exogenous proteins and illuminated their binding specificities.
复合碳水化合物（聚糖）与聚糖结合蛋白的非共价结合介导了许多重要的生理和病理生理过程。识别这些相互作用对于理解其多样的生物学功能至关重要，并有助于开发新的疾病治疗方法和诊断手段。大多数聚糖结合蛋白所识别的聚糖种类及其亲和力的相关知识尚不完整。基于质谱的天然聚糖文库筛选已成为一种有前景的方法，用于定义聚糖结合蛋白的聚糖相互作用组。在此，我们综述了基于质谱的天然文库筛选的最新进展，这些进展促成了对内源性和外源性蛋白质聚糖配体的发现，并阐明了它们的结合特异性。
