Zhang Jingmiao, Xia Ao, Yao Dunxue, Guo Xiaobo, Lam Su Shiung, Huang Yun, Zhu Xianqing, Zhu Xun, Liao Qiang
Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China; Institute of Engineering Thermophysics, School of Energy and Power Engineering, Chongqing University, Chongqing 400044, China.
Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China; Institute of Engineering Thermophysics, School of Energy and Power Engineering, Chongqing University, Chongqing 400044, China.
Bioresour Technol. 2022 Nov;363:127891. doi: 10.1016/j.biortech.2022.127891. Epub 2022 Sep 8.
The development of microalgae-bacteria symbiosis for treating wastewater is flourishing owing to its high biomass productivity and exceptional ability to purify contaminants. A nature-selected microalgae-bacteria symbiosis, mainly consisting of Dictyosphaerium and Pseudomonas, was used to treat oxytetracycline (OTC), ofloxacin (OFLX), and antibiotic-containing swine wastewater. Increased antibiotic concentration gradually reduced biomass productivity and intricately changed symbiosis composition, while 1 mg/L OTC accelerated the growth of symbiosis. The symbiosis biomass productivity reached 3.4-3.5 g/L (5.7-15.3 % protein, 18.4-39.3 % carbohydrate, and 2.1-3.9 % chlorophyll) when cultured in antibiotic-containing swine wastewater. The symbiosis displayed an excellent capacity to remove 76.3-83.4 % chemical oxygen demand, 53.5-62.4 % total ammonia nitrogen, 97.5-100.0 % total phosphorus, 96.3-100.0 % OTC, and 32.8-60.1 % OFLX in swine wastewater. The microbial community analysis revealed that the existence of OTC/OFLX increased the richness and evenness of microalgae but reduced bacteria species in microalgae-bacteria, and the toxicity of OFLX to bacteria was stronger than that of OTC.
微藻-细菌共生体系用于废水处理的发展态势良好，这得益于其高生物量生产力以及出色的污染物净化能力。一种自然选择的微藻-细菌共生体系，主要由盘星藻属和假单胞菌属组成，被用于处理土霉素（OTC）、氧氟沙星（OFLX）以及含抗生素的猪废水。抗生素浓度的增加逐渐降低了生物量生产力，并使共生体系组成发生复杂变化，而1mg/L的土霉素促进了共生体系的生长。当在含抗生素的猪废水中培养时，共生体系的生物量生产力达到3.4-3.5g/L（蛋白质含量为5.7-15.3%，碳水化合物含量为18.4-39.3%，叶绿素含量为2.1-3.9%）。该共生体系在去除猪废水中76.3-83.4%的化学需氧量、53.5-62.4%的总氨氮、97.5-100.0%的总磷、96.3-100.0%的土霉素以及32.8-60.1%的氧氟沙星方面表现出卓越的能力。微生物群落分析表明，土霉素/氧氟沙星的存在增加了微藻的丰富度和均匀度，但减少了微藻-细菌中的细菌种类，且氧氟沙星对细菌的毒性强于土霉素。
