College of Environmental Science and Engineering, Hunan University and Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, Hunan 410082, PR China; Guangdong Provincial Key Laboratory of Petrochemical Pollution Processes and Control, School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming, Guangdong 525000, PR China.
College of Environmental Science and Engineering, Hunan University and Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, Hunan 410082, PR China; Guangdong Provincial Key Laboratory of Petrochemical Pollution Processes and Control, School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming, Guangdong 525000, PR China; Hunan Provincial Environmental Protection Engineering Center for Organic Pollution Control of Urban Water and Wastewater, Changsha, Hunan 410001, PR China; Maoming Engineering Research Center for Organic Pollution Control, Academy of Environmental and Resource Sciences, Guangdong University of Petrochemical Technology, Maoming, Guangdong 525000, PR China.
Bioresour Technol. 2022 Feb;346:126667. doi: 10.1016/j.biortech.2021.126667. Epub 2022 Jan 4.
Removal of nutrients from swine wastewater digester effluent (SWDE) by microalgae Coelastrella sp. and production of Coelastrella sp. were investigated at the presence of oxytetracycline (OTC) and Zn(II). Mechanisms of stress of OTC and Zn(II) on microalgae were discussed via analyzing the removal performance of SWDE and biochemical characteristics of microalgae. Results showed that removal efficiency of ammonia nitrogen and biomass yield of microalgae at the presence of 5000 μg/L of OTC decreased by 13.1% - 50.1% and 28.2% -71.5%, respectively, when Zn concentration was increased from 0.50 mg/L to 5.0 mg/L. The presence of 5.0 mg/L Zn(II) promoted the accumulation of lipids in microalgae, and the presence of 50 μg/L OTC increased unsaturation of fatty acid methyl ester. Content of glutathione and activity of both glutamine synthetase and superoxide dismutase decreased with the increase of OTC concentration, while content of adenosine triphosphatase increased when Zn(II) concentration was also increased.
利用小球藻（Coelastrella sp.）去除猪粪污水消化液（SWDE）中的营养物质，并在氧四环素（OTC）和 Zn(II)存在的条件下生产小球藻。通过分析 SWDE 的去除性能和微藻的生化特性，讨论了 OTC 和 Zn(II)对微藻的胁迫机制。结果表明，当 Zn 浓度从 0.50 mg/L 增加到 5.0 mg/L 时，5000 μg/L OTC 存在下氨氮的去除效率和微藻的生物量产量分别降低了 13.1% - 50.1%和 28.2% -71.5%。5.0 mg/L Zn(II)的存在促进了微藻中脂质的积累，而 50 μg/L OTC 的存在增加了脂肪酸甲酯的不饱和度。谷胱甘肽含量和谷氨酰胺合成酶和超氧化物歧化酶的活性随着 OTC 浓度的增加而降低，而当 Zn(II)浓度增加时，三磷酸腺苷含量增加。
