Pardhiya Sonali, Gautam Rohit, Nirala Jay Prakash, Murmu Nina Nancy, Rajamani Paulraj
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Reprod Toxicol. 2022 Oct;113:136-149. doi: 10.1016/j.reprotox.2022.09.003. Epub 2022 Sep 8.
In recent decades, microwave (MW) radiations are being used extensively for various applications such as Wi-Fi, telecommunication, etc. due to which there have been grave concerns regarding the adverse effects of MW exposure on human health, particularly the reproductive system. MW cause damage to the reproductive system by generating free radicals, decreasing antioxidant defence, and inducing oxidative stress. Hence, the present study was aimed to counteract the harmful effect by using antioxidant enzymes mimicking nanoparticle, Bovine serum albumin (BSA) conjugated manganese dioxide nanoparticle (MNP*). Male Wistar rats were exposed to MW and treated with MNP*, and their individual, as well as combined effect on reproductive parameters was investigated. Results showed that MW exposed rats had significantly reduced testosterone levels along with alterations in the testicular morphology. The antioxidant status decreased, and lipid peroxidation increased significantly in testis. MW exposure also showed altered sperm parameters such as a significant decrease in sperm count, viability, membrane integrity and mitochondrial activity with a significant increase in morphological abnormality and lipid peroxidation. As a result, the changes induced by MW may affect male fertility. However, upon combined exposure of MNP* and MW, these alterations were reduced significantly. Hence, it may be concluded that MNP* could reduce oxidative stress mediated damages in the reproductive system of rats owing to its antioxidant activity, and thus have a potential to act as a radioprotectant.
近几十年来，微波（MW）辐射因其在Wi-Fi、电信等各种应用中的广泛使用，人们对微波暴露对人体健康，尤其是生殖系统的不利影响深感担忧。微波通过产生自由基、降低抗氧化防御能力和诱导氧化应激来损害生殖系统。因此，本研究旨在通过使用模拟纳米颗粒的抗氧化酶——牛血清白蛋白（BSA）共轭二氧化锰纳米颗粒（MNP*）来抵消这种有害影响。将雄性Wistar大鼠暴露于微波并给予MNP处理，研究它们对生殖参数的单独及联合影响。结果表明，暴露于微波的大鼠睾酮水平显著降低，同时睾丸形态发生改变。睾丸中的抗氧化状态下降，脂质过氧化显著增加。微波暴露还显示精子参数发生改变，如精子数量、活力、膜完整性和线粒体活性显著降低，形态异常和脂质过氧化显著增加。因此，微波引起的这些变化可能会影响雄性生育能力。然而，在MNP和微波联合暴露后，这些改变显著减少。因此，可以得出结论，MNP*由于其抗氧化活性，可以减少微波介导的对大鼠生殖系统的损伤，因此有潜力作为一种辐射防护剂。
