Hosseini Leila, Majdi Alireza, Sadigh-Eteghad Saeed, Farajdokht Fereshteh, Ziaee Mojtaba, Rahigh Aghsan Sepideh, Farzipour Mohammad, Mahmoudi Javad
Research Center of Psychiatry and Behavioral Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Exp Gerontol. 2022 Oct 15;168:111950. doi: 10.1016/j.exger.2022.111950. Epub 2022 Sep 8.
The behavioral effects and molecular signaling mechanisms of Coenzyme Q (Q) in age-related memory impairment are poorly understood. This study aimed to investigate the effects of Q on memory impairment, oxidative stress, apoptosis, and mitophagy in aged rats. 40 aged (24 months old) and 10 young (3 months old) male Wistar rats were randomly divided into the following groups (n = 10/group): young + vehicle, aged + vehicle, and aged + Q (at 100, 200, 300 mg/kg/day doses). Treatments were administrated orally by gavage for 2 weeks. The novel object recognition test was used to assess episodic memory. Oxidative stress, apoptosis, and mitophagy-related protein expressions were measured in the hippocampus. We found that Q10 reversed aging-induced memory impairment at the dose of 300 mg/kg. Moreover, aging was associated with a reduction in ATP production, decrease in mitophagy-related proteins (PINK, Parkin, and P62 levels and LC3II/I ratio), excessive generation of reactive oxygen species and lipid peroxidation, and apoptosis in the hippocampus, which were partially reversed following oral administration of Q. These findings indicate the therapeutic potential of Q in aging-induced memory decline.
辅酶Q（Q）在年龄相关性记忆障碍中的行为效应和分子信号传导机制尚不清楚。本研究旨在探讨Q对老年大鼠记忆障碍、氧化应激、细胞凋亡和线粒体自噬的影响。将40只老年（24月龄）和10只年轻（3月龄）雄性Wistar大鼠随机分为以下几组（每组n = 10）：年轻+赋形剂组、老年+赋形剂组和老年+Q组（剂量分别为100、200、300 mg/kg/天）。通过灌胃口服给药2周。采用新物体识别试验评估情景记忆。检测海马中氧化应激、细胞凋亡和线粒体自噬相关蛋白的表达。我们发现，300 mg/kg剂量的辅酶Q10可逆转衰老诱导的记忆障碍。此外，衰老与ATP生成减少、线粒体自噬相关蛋白（PINK、Parkin和P62水平以及LC3II/I比值）降低、活性氧过度生成和脂质过氧化以及海马细胞凋亡有关，口服Q后这些情况得到部分逆转。这些发现表明Q在衰老诱导的记忆衰退中具有治疗潜力。
