ICAR-Indian Veterinary Research Institute, Hebbal, Karnataka, 560024, Bengaluru, India.
ICAR-CIRB, Sirsa Rd., Haryana, 125001, Hisar, India.
Appl Microbiol Biotechnol. 2022 Oct;106(19-20):6745-6757. doi: 10.1007/s00253-022-12151-2. Epub 2022 Sep 12.
Large-scale monitoring of foot-and-mouth disease (FMD) in livestock is imperative in an FMD control program. Detection of antibodies against non-structural proteins (NSP) of FMD virus (FMDV) is one of the best tools to estimate the prevalence of past infection; availability of such a well-validated test is therefore essential. Using a FMDV 3B protein-specific monoclonal antibody, we have developed a new NSP antibody blocking ELISA (10H9 bELISA) and validated it on large panels of sera from different susceptible species. The diagnostic sensitivity of the ELISA was 95% with a specificity of 98%, similar to the values found using a commercial kit (PrioCHECK FMD NS test). The 10H9 bELISA can be used in a broad range of FMD susceptible species making it a very useful tool in monitoring the foot-and-mouth disease control programs by detection of virus circulation in the vaccinated populations. KEY POINTS: • A new ELISA for detection of foot and mouth disease (FMD) antibodies. • Diagnostic sensitivity of 95% and specificity of 98%. • Tested with panels of validated sera from broad host range.
大规模监测家畜口蹄疫（FMD）在口蹄疫防控计划中至关重要。检测针对口蹄疫病毒（FMDV）非结构蛋白（NSP）的抗体是估计过去感染流行率的最佳工具之一；因此，这种经过充分验证的测试的可用性是必不可少的。我们使用口蹄疫 3B 蛋白特异性单克隆抗体，开发了一种新的 NSP 抗体阻断 ELISA（10H9 bELISA），并在来自不同易感物种的大量血清样本中对其进行了验证。该 ELISA 的诊断灵敏度为 95%，特异性为 98%，与使用商业试剂盒（PrioCHECK FMD NS test）获得的值相似。10H9 bELISA 可用于广泛的 FMD 易感物种，使其成为通过检测疫苗接种人群中的病毒传播来监测口蹄疫控制计划的非常有用的工具。关键点：
• 一种用于检测口蹄疫（FMD）抗体的新 ELISA。
• 诊断灵敏度为 95%，特异性为 98%。
• 用广泛宿主范围的经验证血清样本进行了测试。
