Jin Yanlong, Chen Shiqiang, Xu Xiao, Jiang Congcong, He Zhentian, Shen Huiquan, Ji Wanquan, Yang Ping
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, China.
Plant Dis. 2023 Apr;107(4):1044-1053. doi: 10.1094/PDIS-04-22-0760-RE. Epub 2023 Apr 19.
Soilborne pathogens destabilize the yields of crops, including barley ( L.) and wheat ( L.). Although genetic resistance derived from relatives of these species has been utilized to prevent rust diseases (i.e., in the wheat-rye 1BL-1RS translocation line), research on resistance against soilborne pathogens remains limited. Here, we performed field trials using 76 genotypes representing 28 , six , and two species to examine resistance against three soilborne bymoviruses: barley yellow mosaic virus (BaYMV), barley mild mosaic virus (BaMMV), and wheat yellow mosaic virus (WYMV). We also performed greenhouse tests using the soilborne fungal pathogen , which causes Fusarium crown rot (FCR). Using RT-PCR, we detected BaMMV and BaYMV in several species, whereas WYMV induced systemic infection in the and species. The identification of FCR susceptibility in all species examined suggests that is a facultative fungal pathogen in . Intraspecies variation in FCR disease severity was observed for several species, pointing to the possibility of exploring host resistance mechanisms. Therefore, by unlocking the host specificity of four soilborne pathogens in species and their relatives, we obtained insights for the further exploration of wild sources of soilborne pathogen resistance for future wheat and barley improvement programs.
土传病原体使包括大麦（L.）和小麦（L.）在内的作物产量不稳定。尽管已利用这些物种亲缘种的遗传抗性来预防锈病（即小麦 - 黑麦1BL - 1RS易位系），但针对土传病原体抗性的研究仍然有限。在这里，我们使用代表28个、6个和2个物种的76个基因型进行了田间试验，以检测对三种土传大麦黄花叶病毒的抗性：大麦黄花叶病毒（BaYMV）、大麦轻度花叶病毒（BaMMV）和小麦黄花叶病毒（WYMV）。我们还使用引起镰刀菌冠腐病（FCR）的土传真菌病原体进行了温室试验。通过逆转录 - 聚合酶链反应（RT - PCR），我们在几个物种中检测到了BaMMV和BaYMV，而WYMV在和物种中引起了系统感染。在所检测的所有物种中均鉴定出对FCR敏感，这表明是中的一种兼性真菌病原体。在几个物种中观察到了FCR病害严重程度的种内变异，这表明有可能探索宿主抗性机制。因此，通过揭示物种及其亲缘种中四种土传病原体的宿主特异性，我们获得了一些见解，以便为未来的小麦和大麦改良计划进一步探索土传病原体抗性的野生资源。
