尿路致病性分离株的毒力标志物鉴定、系统发育群关联及抗菌药物敏感性分析

Identification of Virulence Markers and Phylogenetic Groups' Association, and Antimicrobial Susceptibility of Uropathogenic Isolates.

作者信息

Meziani Dahbia Yasmina, Barnich Nicolas, Boucheham Anouar, Rezgoune Mohamed Larbi, Benlabed Kaddour, Rodrigues Michael, Satta Dalila

机构信息

Molecular and Cellular Biology Laboratory, Frères Mentouri Constantine I University, B.P. 325, Ain El Bey Road, Constantine, 25000, Constantine, Algeria.

University Clermont Auvergne, M2iSH, UMR 1071 INSERM, INRA USC 2018, Clermont-Ferrand, 63001, France.

出版信息

Infect Disord Drug Targets. 2023;23(2):e080922208695. doi: 10.2174/1871526522666220908161529.

DOI:10.2174/1871526522666220908161529

PMID:36089795

Abstract

BACKGROUND

Urinary tract infections represent a world public health problem, which is caused mainly by Uropathogenic Escherichia coli. Although they are originally found in the intestinal microbiota in the majority of the cases, urinary tract infections can also be caused by intra-intestinal pathogenic E. coli.

OBJECTIVE

The main objective of our research is to identify the virulence factors generally associated with different pathotypes across phylogenetic groups.

METHODS

E. coli were isolated from patients with urinary tract infections. Antimicrobial susceptibility tests, virulence genes and phylogroups were prospected. The data analysis were performed using the chi-square and Fisher exact test.

RESULTS

In total, 72.2% of isolates showed multidrug resistant. We have also depicted an important association between E. coli from inpatients with UTIs and pap and hlyA genes (p-0.041 and p-0.019 respectively). The predominant phylogenetic group in our isolates is B2 (45.4%) followed by D (12.4%). Our results showed that 9.3% of isolates have an unknown phylogroup which shows a significant association with astA gene (p-0.008). We have as well found a significant association between B2 and three virulence genes namely pap, hlyA and invE (p-0.002, p-0.001, p-0.025 respectively); B1 and pap, hlyA genes (p-0.049 and p-0.021 respectively); E and afa gene (p-0.024).

CONCLUSION

Certain virulence factors have been shown to be potential targets for drug design and therapeutic pathways in order to deal with the antimicrobial resistance problem enhanced by antibiotic therapy.

摘要

背景

尿路感染是一个全球公共卫生问题，主要由尿路致病性大肠杆菌引起。虽然大多数情况下它们最初存在于肠道微生物群中，但尿路感染也可能由肠道内致病性大肠杆菌引起。

目的

我们研究的主要目的是确定与不同系统发育组中不同致病型普遍相关的毒力因子。

方法

从尿路感染患者中分离出大肠杆菌。进行了药敏试验、毒力基因和系统发育组分析。使用卡方检验和Fisher精确检验进行数据分析。

结果

总共72.2%的分离株显示出多重耐药性。我们还描述了住院尿路感染患者的大肠杆菌与pap和hlyA基因之间的重要关联（分别为p = 0.041和p = 0.019）。我们分离株中占主导地位的系统发育组是B2（45.4%），其次是D（12.4%）。我们的结果表明，9.3%的分离株具有未知的系统发育组，这与astA基因显示出显著关联（p = 0.008）。我们还发现B2与三个毒力基因pap、hlyA和invE之间存在显著关联（分别为p = 0.002、p = 0.001、p = 0.025）；B1与pap、hlyA基因之间存在显著关联（分别为p = 0.049和p = 0.021）；E与afa基因之间存在显著关联（p = 0.024）。