College of Animal Science and Technology, Guangxi University, Nanning, China.
Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland, Yancheng Institute of Technology, Yancheng, China.
Front Immunol. 2022 Aug 24;13:974310. doi: 10.3389/fimmu.2022.974310. eCollection 2022.
Mammalian single immunoglobulin (Ig) interleukin-1 receptor related molecule (SIGIRR), an important member of the Toll/interleukin-1 receptor (TIR) family, plays important balancing roles in the inflammatory responses. In the present study, the double Ig interleukin-1 receptor related molecule (DIGIRR), the homologous of SIGIRR, was characterized in golden pompano () (termed as trDIGIRR). The full-length cDNA of trDIGIRR was 2,167 bp with an open reading frame (ORF) of 1,572 bp encoding 523 amino acids. The trDIGIRR contained several conserved domains including a signal peptide, two Ig domains, a transmembrane domain and a TIR domain, and shared high sequence identities with its teleost counterparts. Realtime qPCR analysis revealed that the trDIGIRR was distributed in all tissues examined, with high expressions in intestine, liver and head kidney. The expressions of trDIGIRR were induced by , lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly I:C) challenge. Further analysis revealed that trDIGIRR was mainly located in the cytoplasm. In addition, the co-immunoprecipitation (co-IP) assay identified that trDIGIRR could interact with myeloid differentiation factor 88 (MyD88), but not interact with TIR domain containing adaptor protein inducing interferon-β (TRIF). Our results provide basis for studying the immune role of fish DIGIRR.
哺乳动物单免疫球蛋白 (Ig) 白细胞介素-1 受体相关分子 (SIGIRR) 是 Toll/白细胞介素-1 受体 (TIR) 家族的重要成员，在炎症反应中发挥着重要的平衡作用。本研究中，鉴定了金黄色鲅 ( ) 的双免疫球蛋白白细胞介素-1 受体相关分子 (DIGIRR)，即 SIGIRR 的同源物 (trDIGIRR)。trDIGIRR 的全长 cDNA 为 2167bp，开放阅读框 (ORF) 为 1572bp，编码 523 个氨基酸。trDIGIRR 包含几个保守结构域，包括信号肽、两个 Ig 结构域、跨膜结构域和 TIR 结构域，与它的硬骨鱼对应物具有高度的序列同一性。实时 qPCR 分析显示，trDIGIRR 分布在所有检测的组织中，在肠、肝和头肾中表达量较高。trDIGIRR 的表达受 、脂多糖 (LPS) 和多聚肌苷酸-多聚胞苷酸 (poly I:C) 刺激诱导。进一步分析显示，trDIGIRR 主要位于细胞质中。此外，共免疫沉淀 (co-IP) 分析鉴定 trDIGIRR 可以与髓样分化因子 88 (MyD88) 相互作用，但不能与 TIR 结构域包含的衔接蛋白诱导干扰素-β (TRIF) 相互作用。我们的结果为研究鱼类 DIGIRR 的免疫作用提供了基础。
