Cleverdon Riley E G, Braun Jessica L, Geromella Mia S, Whitley Kennedy C, Marko Daniel M, Hamstra Sophie I, Roy Brian D, MacPherson Rebecca E K, Fajardo Val A
Department of Kinesiology, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON, L2S 3A1, Canada.
Centre for Bone and Muscle Health, Brock University, St. Catharines, ON, Canada.
iScience. 2022 Aug 18;25(9):104972. doi: 10.1016/j.isci.2022.104972. eCollection 2022 Sep 16.
The DBA/2J (D2) mouse is a more severe model of Duchenne muscular dystrophy when compared to the traditional C57BL/10 (C57) mouse. Here, we questioned whether sarco(endo)plasmic reticulum Ca-ATPase (SERCA) function would differ in muscles from young D2 and C57 mice. Both D2 and C57 mice exhibited signs of impaired Ca uptake in the gastrocnemius, diaphragm, and left ventricle; however, the level of impairment was more severe in D2 mice. Reductions in maximal SERCA activity were also more prominent in the D2 gastrocnemius and diaphragm when compared to those from C57 mice; however, there were no differences detected in the left ventricle. Across all muscles, D2 mice had the highest levels of oxidative stress as indicated by protein nitrosylation and/or nitration. In conclusion, our study shows that SERCA function is more impaired in young D2 mice compared with age-matched C57 mice.
与传统的C57BL/10（C57）小鼠相比，DBA/2J（D2）小鼠是杜氏肌营养不良症更严重的模型。在此，我们质疑肌浆（内质）网Ca-ATP酶（SERCA）功能在年轻D2和C57小鼠的肌肉中是否会有所不同。D2和C57小鼠在腓肠肌、膈肌和左心室中均表现出钙摄取受损的迹象；然而，D2小鼠的受损程度更严重。与C57小鼠相比，D2腓肠肌和膈肌中SERCA最大活性的降低也更显著；然而，左心室中未检测到差异。在所有肌肉中，蛋白质亚硝化和/或硝化表明D2小鼠的氧化应激水平最高。总之，我们的研究表明，与年龄匹配的C57小鼠相比，年轻D2小鼠的SERCA功能受损更严重。
